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Cat.No. : CSC-RI0075 Host Cell: CHO
| Cat. No. | CSC-RI0075 |
| Description | This cell line is engineered to overexpress human CACNA2D1. |
| Background | The human CACNA1B gene encodes the pore-forming subunit of Cav2.2, the voltage-gated N-type calcium channel. CACNB3 and CACNA2D1 encode auxiliary subunits that modulate gating and pharmacological properties. Cav2.2 channels expressed in CNS neurons are therapeutic targets in pain. |
| Gene | CACNA2D1 |
| Gene Species | Homo sapiens (Human) |
| Alias | CACNB3, CACNLB3, CAB3, FLJ58949 |
| Host Cell | CHO |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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