Human CACNA1B Stable Cell Line-HEK293

Name: Human CACNA1B Stable Cell Line-HEK293
SKU: CSC-RI0001
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0001

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0001
Description	This cell line is engineered to overexpress human CACNA1B.
Background	CACNA1B is a voltage-gated calcium channel alpha subunit. It is expressed almost exclusively in neurons typically located at high densities in presynaptic terminals. Opening of these channels enables the rapid influx of the extracellular Ca2+, triggering exocytotic vesicle fusion with terminal membranes and subsequent neurotransmitter release. Evidence suggests that Cav2.2 channels have a fundamental role in instigating increased excitability and neurotransmitter release in various pain syndromes. The link between CACNA1B and pain neurotransmission in humans has been further vindicated by the effectiveness of PrialtR (ziconotide), a synthetic version of omega-conotoxin MVIIA, in treating severe chronic pain.
Target Gene	CACNA1B
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuCACNA1B
Alias	BHLHE22, BHLHB5, TNRC20, Beta3, bHLHe22, CAGL85
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CACNA1B calcium channel, voltage-dependent, N type, alpha 1B subunit [ Homo sapiens ]
Gene Symbol	CACNA1B
Synonyms	BIII; CACNN; Cav2.2; CACNL1A5
GeneID	774
Uni ProtID	Q00975
mRNA Refseq	NM_000718.3
Protein Refseq	NP_000709.1
Chromosome Location	9q34
Function	ATP binding; protein C-terminus binding; protein binding; voltage-gated calcium channel activity;
Pathway	Calcium Regulation in the Cardiac Cell, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Cholinergic synapse, organism-specific biosystem; Depolarization of the Presynaptic Terminal Triggers the Opening of Calcium Channels, organism-specific biosystem; Dopaminergic synapse, organism-specific biosystem; Dopaminergic synapse, conserved biosystem;
MIM	601012

Quick Inquiry

Q & A

Customer Reviews

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *