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Cat.No. : CSC-RI0068 Host Cell: CHO
| Cat. No. | CSC-RI0068 |
| Description | This cell line is engineered to overexpress human CACNA2D1. |
| Background | The human CACNA1C gene encodes the pore-forming subunits of Cav1.2. CACNB2 and CACNA2D1 encode auxiliary subunits that modulate gating and pharmacological properties. Cav1.2 channels expressed in heart, smooth muscle, neurons, and endocrine tissue are therapeutic targets in cardiac arrhythmia and hypertension. |
| Gene | CACNA2D1 |
| Gene Species | Homo sapiens (Human) |
| Alias | CACNB2, CACNLB2, MYSB, CAB2, MYSB, CAVB2, CACNLB2, FLJ23743 |
| Host Cell | CHO |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
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