Human CACNA1C/CACNB2/CACNA2D1 Stable Cell Line-HEK293

Name: Human CACNA1C/CACNB2/CACNA2D1 Stable Cell Line-HEK293
SKU: CSC-RI0067
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0067

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0067
Description	This cell line is engineered to overexpress human CACNA2D1.
Background	The human CACNA1C gene encodes the pore-forming subunit of voltage-gated L-type Ca2+ channels. Co-expression with the beta 2 and alpha2delta1 auxiliary subunits recapitulates function in heterologous expression (CHO cells). Cav1.2 channels, widely distributed in cardiac and smooth muscle, and in neurons, are therapeutic targets in cardiovascular and neurological diseases.
Target Gene	CACNA2D1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuCACNA1C/CACNB2/CACNA2D1
Alias	CACNB2, CACNLB2, MYSB, CAB2, MYSB, CAVB2, CACNLB2, FLJ23743
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CACNA2D1 calcium channel, voltage-dependent, alpha 2/delta subunit 1 [ Homo sapiens ]
Gene Symbol	CACNA2D1
Synonyms	CACNA2; CCHL2A; CACNL2A
Gene Description	calcium channel, voltage-dependent, alpha 2/delta subunit 1
GeneID	781
Uni ProtID	P54289
mRNA Refseq	NM_000722.2
Protein Refseq	NP_000713.2
Chromosome Location	7q21-q22
Function	metal ion binding; voltage-gated calcium channel activity; contributes_to voltage-gated calcium channel activity;
Pathway	Arrhythmogenic right ventricular cardiomyopathy, organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), organism-specific biosystem; Arrhythmogenic right ventricular cardiomyopathy (ARVC), conserved biosystem; Cardiac muscle contraction, organism-specific biosystem; Cardiac muscle contraction, conserved biosystem; Depolarization of the Presynaptic Terminal Triggers the Opening of Calcium Channels, organism-specific biosystem; Dilated cardiomyopathy, organism-specific biosystem;
MIM	114204

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