Human CACNA1H Stable Cell Line-HEK293

Name: Human CACNA1H Stable Cell Line-HEK293
SKU: CSC-RI0002
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0002

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0002
Description	This cell line is engineered to overexpress human CACNA1H.
Background	The T-type calcium channel is a type of voltage-dependent calcium channel, the alpha1 subunit determines most of the channel"s properties. The T-type calcium channel is one of the components that produce the pacemaker potential in the SA node of the heart. T-type calcium channel blockers are used primarily as antiepileptics. Two voltage-dependent calcium channels play critical roles in the physiology of cardiac muscle: L-type calcium channel and T-type calcium channels voltage-gated calcium channels. L-type channels are important in sustaining an action potential, while T-type channels are important in initiating them.
Target Gene	CACNA1H
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuCACNA1H
Alias	CACNA1H, Cav3.2, ECA6, EIG6, CACNA1HB, FLJ90484
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	CACNA1H calcium channel, voltage-dependent, T type, alpha 1H subunit [ Homo sapiens ]
Gene Symbol	CACNA1H
Synonyms	ECA6; EIG6; Cav3.2; CACNA1HB
Gene Description	calcium channel, voltage-dependent, T type, alpha 1H subunit
GeneID	8912
Uni ProtID	B3KQH9
mRNA Refseq	NM_001005407.1
Protein Refseq	NP_001005407.1
Chromosome Location	16p13.3
Function	low voltage-gated calcium channel activity; metal ion binding; scaffold protein binding;
Pathway	Axon guidance, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Developmental Biology, organism-specific biosystem; MAPK signaling pathway, organism-specific biosystem; MAPK signaling pathway, conserved biosystem; NCAM signaling for neurite out-growth, organism-specific biosystem;
MIM	607904

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Q & A

Customer Reviews

Customer Q&As

How should Human CACNA1H Stable Cell Line-HEK293 cells be revived upon receipt?

A: For cells shipped on dry ice, if the dry ice has completely melted, immediately recover and culture the cells. Do not refreeze. If there is still dry ice, either recover and culture the cells or place the cryovial containing the cells in liquid nitrogen for storage, but do not store the cells in a high-temperature environment.

What are the cell culture conditions for Human CACNA1H Stable Cell Line-HEK293?

A: Human CACNA1H Stable Cell Line-HEK293 should be cultured at 37°C with 5% CO2.

What drugs is Human CACNA1H Stable Cell Line-HEK293 sensitive to?

A: This cell line is sensitive to ω-conotoxin GVIA and ω-agatoxin IVA but not sensitive to dihydropyridines.

What is the culture medium for the Human CACNA1H Stable Cell Line-HEK293?

A: The culture medium for Human CACNA1H Stable Cell Line-HEK293 is DMEM + 10% FBS. Add an appropriate amount of penicillin-streptomycin solution to the culture medium to prevent potential bacterial contamination.

What is the CACNA1H gene?

A: The human CACNA1H gene encodes the pore-forming subunit of Cav3.2, a voltage-gated T-type calcium channel.

What are the storage conditions for Human CACNA1H Stable Cell Line-HEK293?

A: For cells shipped in culture flasks or centrifuge tubes, they are effective at room temperature for 3-5 days. For cells shipped on dry ice, store them in liquid nitrogen for long-term storage.

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