Cat.No. : CSC-RI0002 Host Cell: HEK293
| Cat. No. | CSC-RI0002 |
| Description | This cell line is engineered to overexpress human CACNA1H. |
| Background | The T-type calcium channel is a type of voltage-dependent calcium channel, the alpha1 subunit determines most of the channel"s properties. The T-type calcium channel is one of the components that produce the pacemaker potential in the SA node of the heart. T-type calcium channel blockers are used primarily as antiepileptics. Two voltage-dependent calcium channels play critical roles in the physiology of cardiac muscle: L-type calcium channel and T-type calcium channels voltage-gated calcium channels. L-type channels are important in sustaining an action potential, while T-type channels are important in initiating them. |
| Gene | CACNA1H |
| Gene Species | Homo sapiens (Human) |
| Alias | CACNA1H, Cav3.2, ECA6, EIG6, CACNA1HB, FLJ90484 |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: For cells shipped on dry ice, if the dry ice has completely melted, immediately recover and culture the cells. Do not refreeze. If there is still dry ice, either recover and culture the cells or place the cryovial containing the cells in liquid nitrogen for storage, but do not store the cells in a high-temperature environment.
A: Human CACNA1H Stable Cell Line-HEK293 should be cultured at 37°C with 5% CO2.
A: This cell line is sensitive to ω-conotoxin GVIA and ω-agatoxin IVA but not sensitive to dihydropyridines.
A: The culture medium for Human CACNA1H Stable Cell Line-HEK293 is DMEM + 10% FBS. Add an appropriate amount of penicillin-streptomycin solution to the culture medium to prevent potential bacterial contamination.
A: The human CACNA1H gene encodes the pore-forming subunit of Cav3.2, a voltage-gated T-type calcium channel.
A: For cells shipped in culture flasks or centrifuge tubes, they are effective at room temperature for 3-5 days. For cells shipped on dry ice, store them in liquid nitrogen for long-term storage.
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