Cat.No. : CSC-RG0040 Host Cell: HEK293
| Cat. No. | CSC-RG0040 |
| Gene | AGTR1 |
| Gene Species | Homo sapiens (Human) |
| Alias | AGTR1, AGTR1B, AG2S, AGTR1A, AT1, AT1B, AT2R1, AT2R1A, AT2R1B, HAT1R, AT1AR, AT1BR, AT1R, AGTR1B |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Morphology | Epithelial |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: To ensure the functionality and stability of the SNAP tag, it is crucial to first verify the insertion site and sequence correctness through PCR and sequence analysis. Secondly, the expression and localization of the SNAP tag can be assessed through immunofluorescence staining and Western Blot. Additionally, the activity of the SNAP tag can be evaluated through functional assays, such as SNAP-Tag substrate binding assays. During long-term culture, it is necessary to regularly monitor the expression levels of the SNAP tag to ensure its stability.
A: This cell line can be used for screening and evaluating potential drugs targeting the AGTR1 receptor by monitoring the interaction between the SNAP tag and drugs or other small molecules. This can be achieved through techniques such as fluorescence resonance energy transfer (FRET) or co-immunoprecipitation (Co-IP), providing a high-throughput screening platform for drug development.
A: The culture conditions can be optimized by adjusting the composition of the growth medium, such as adding appropriate growth factors and nutrients, and adjusting environmental factors like pH and temperature. Regular medium changes and avoiding overcrowding can help maintain cell health, which in turn can improve the expression of the SNAP tag.
A: This cell line, by expressing the AGTR1 receptor with a SNAP tag, allows researchers to monitor the interaction between angiotensin II and its receptor in living cells in real-time, as well as subsequent signaling events. This is crucial for understanding the mechanism of action of angiotensin II in cardiovascular diseases.
A: By using the AGTR1 receptor with a SNAP tag, researchers can employ biochemical and biophysical methods, such as surface plasmon resonance (SPR) or single-molecule imaging techniques, to study the activation process of GPCRs. These technologies can provide detailed information about receptor conformational changes and G protein activation.
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