Human AGTR1-SNAP Stable Cell Line-HEK293

Name: Human AGTR1-SNAP Stable Cell Line-HEK293
SKU: CSC-RG0040
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0040

Host Cell : HEK293 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0040
Target Gene	AGTR1
Gene Species	Homo sapiens (Human)
Abbr	HEK293-HuAGTR1-SNAP
Alias	AGTR1, AGTR1B, AG2S, AGTR1A, AT1, AT1B, AT2R1, AT2R1A, AT2R1B, HAT1R, AT1AR, AT1BR, AT1R, AGTR1B
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Morphology	Epithelial

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	AGTR1 angiotensin II receptor, type 1 [ Homo sapiens ]
Gene Symbol	AGTR1
Synonyms	AT1; AG2S; AT1B; AT1R; AT1AR; AT1BR; AT2R1; HAT1R; AGTR1A; AGTR1B; AT2R1A; AT2R1B
Gene Description	angiotensin II receptor, type 1
Gene ID	185
Uni Prot ID	P30556
m RNA Refseq	NM_000685.4
Protein Refseq	NP_000676.1
Chromosome Location	3q24
Function	acetyltransferase activator activity; angiotensin type I receptor activity; angiotensin type I receptor activity; angiotensin type II receptor activity; bradykinin receptor binding; protein binding; protein heterodimerization activity;
Pathway	ACE Inhibitor Pathway, organism-specific biosystem; Angiopoietin receptor Tie2-mediated signaling, organism-specific biosystem; Arf6 signaling events, organism-specific biosystem; Calcium signaling pathway, organism-specific biosystem; Calcium signaling pathway, conserved biosystem; Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (q) signalling events, organism-specific biosystem;
MIM	106165

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Background

Q & A

Customer Reviews

Angiotensin II is a potent vasopressor hormone and a primary regulator of aldosterone secretion. It is an important effector controlling blood pressure and volume in the cardiovascular system. It acts through at least two types of receptors. This gene encodes the type 1 receptor which is thought to mediate the major cardiovascular effects of angiotensin II. This gene may play a role in the generation of reperfusion arrhythmias following restoration of blood flow to ischemic or infarcted myocardium. It was previously thought that a related gene, denoted as AGTR1B, existed; however, it is now believed that there is only one type 1 receptor gene in humans. Multiple alternatively spliced transcript variants have been reported for this gene.

Customer Q&As

How can the functionality and stability of the SNAP tag in the Human AGTR1-SNAP Stable Cell Line-HEK293 be ensured?

A: To ensure the functionality and stability of the SNAP tag, it is crucial to first verify the insertion site and sequence correctness through PCR and sequence analysis. Secondly, the expression and localization of the SNAP tag can be assessed through immunofluorescence staining and Western Blot. Additionally, the activity of the SNAP tag can be evaluated through functional assays, such as SNAP-Tag substrate binding assays. During long-term culture, it is necessary to regularly monitor the expression levels of the SNAP tag to ensure its stability.

What is the application of the Human AGTR1-SNAP Stable Cell Line-HEK293 in drug screening?

A: This cell line can be used for screening and evaluating potential drugs targeting the AGTR1 receptor by monitoring the interaction between the SNAP tag and drugs or other small molecules. This can be achieved through techniques such as fluorescence resonance energy transfer (FRET) or co-immunoprecipitation (Co-IP), providing a high-throughput screening platform for drug development.

How can the culture conditions for the Human AGTR1-SNAP Stable Cell Line-HEK293 be optimized to enhance cell growth rate and SNAP tag expression?

A: The culture conditions can be optimized by adjusting the composition of the growth medium, such as adding appropriate growth factors and nutrients, and adjusting environmental factors like pH and temperature. Regular medium changes and avoiding overcrowding can help maintain cell health, which in turn can improve the expression of the SNAP tag.

What is the role of the Human AGTR1-SNAP Stable Cell Line-HEK293 in studying the angiotensin II signaling pathway?

A: This cell line, by expressing the AGTR1 receptor with a SNAP tag, allows researchers to monitor the interaction between angiotensin II and its receptor in living cells in real-time, as well as subsequent signaling events. This is crucial for understanding the mechanism of action of angiotensin II in cardiovascular diseases.

How can the activation mechanism of G protein-coupled receptors (GPCRs) be studied using the Human AGTR1-SNAP Stable Cell Line-HEK293?

A: By using the AGTR1 receptor with a SNAP tag, researchers can employ biochemical and biophysical methods, such as surface plasmon resonance (SPR) or single-molecule imaging techniques, to study the activation process of GPCRs. These technologies can provide detailed information about receptor conformational changes and G protein activation.

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