Human ACCN2 Stable Cell Line-CHO

Name: Human ACCN2 Stable Cell Line-CHO
SKU: CSC-RI0062
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RI0062

Host Cell : CHO Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RI0062
Description	This cell line is engineered to overexpress human ASIC1.
Background	The human ACCN2 gene encodes the pore-forming subunit of ASIC1a, a proton-activated, channel permeable to both Na+ and Ca2+ ions. ASIC1a channels widely expressed in the neurons of peripheral sensory and the central nervous system are therapeutic targets in pain and neurodegenerative diseases.
Target Gene	ASIC1
Gene Species	Homo sapiens (Human)
Abbr	CHO-HuACCN2
Alias	ASIC1, ACCN2, ASIC, BNaC2
Host Cell	CHO
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Homo sapiens acid-sensing (proton-gated) ion channel 1 DNA.
Gene Symbol	ASIC1
Synonyms	ASIC, ACCN2, BNaC2
m RNA Refseq	NM_001095.3

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Q & A

Customer Reviews

Customer Q&As

How is the specific expression level of the Human ACCN2 Stable Cell Line-CHO quantified?

A: The expression level of ACCN2 in the cell line can be quantified using Western Blot and quantitative PCR (qPCR) to ensure appropriate expression under physiological conditions.

How does the Human ACCN2 Stable Cell Line-CHO simulate the human body environment in drug testing?

A: By adding specific cytokines or signaling molecules to the cell culture, the microenvironment of the human body can be simulated for more accurate assessment of drugs' effects on ACCN2 channels.

What considerations are there when using this cell line in electrophysiological experiments?

A: Particular attention should be paid to the electrical characteristics of the ACCN2 channel, such as current amplitude and action potential duration.

How could the Human ACCN2 Stable Cell Line-CHO be useful in research on rare diseases?

A: This cell line might be particularly useful in studying rare genetic neuropathic conditions related to mutations in ACCN2, such as certain forms of hereditary pain syndromes.

How can experimental errors be minimized when conducting research with this cell line?

A: By strictly controlling experimental conditions, such as temperature, pH, and drug concentration, and by replicating experiments to ensure the reliability of results and reduce errors.

What are the unique applications of this cell line in sensory biology research?

A: Due to the key role of ACCN2 in the activation of sensory neurons, this cell line is particularly suited for studying mechanisms of touch and temperature perception.

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