Cat.No. : CSC-RI0062 Host Cell: CHO
| Cat. No. | CSC-RI0062 |
| Description | This cell line is engineered to overexpress human ASIC1. |
| Background | The human ACCN2 gene encodes the pore-forming subunit of ASIC1a, a proton-activated, channel permeable to both Na+ and Ca2+ ions. ASIC1a channels widely expressed in the neurons of peripheral sensory and the central nervous system are therapeutic targets in pain and neurodegenerative diseases. |
| Gene | ASIC1 |
| Gene Species | Homo sapiens (Human) |
| Alias | ASIC1, ACCN2, ASIC, BNaC2 |
| Host Cell | CHO |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Morphology | Epithelial-like |
| Stability | Validated for at least 10 passages |
| Application | 1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Channelopathies research |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Adherent |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The expression level of ACCN2 in the cell line can be quantified using Western Blot and quantitative PCR (qPCR) to ensure appropriate expression under physiological conditions.
A: By adding specific cytokines or signaling molecules to the cell culture, the microenvironment of the human body can be simulated for more accurate assessment of drugs' effects on ACCN2 channels.
A: Particular attention should be paid to the electrical characteristics of the ACCN2 channel, such as current amplitude and action potential duration.
A: This cell line might be particularly useful in studying rare genetic neuropathic conditions related to mutations in ACCN2, such as certain forms of hereditary pain syndromes.
A: By strictly controlling experimental conditions, such as temperature, pH, and drug concentration, and by replicating experiments to ensure the reliability of results and reduce errors.
A: Due to the key role of ACCN2 in the activation of sensory neurons, this cell line is particularly suited for studying mechanisms of touch and temperature perception.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
