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Cat. No. : PTAK-037
| Cat. No. | PTAK-037 |
| Description | The UCH-L3 Activity Assay Kit utilizes the ability of active human UCH-L3 deubiquitinase to cleave a synthetic DUB substrate to release a fluorophore, which can be easily quantified (Ex/Em = 360/460 nm) using a fluorescence microplate reader. This inhibitor screening kit thus allows rapid and reliable determination of the inhibitory effects of various compounds on UCH-L3 deubiquitinase and could be used to screen for novel inhibitors. |
| Component |
•UCH-L3 assay buffer •UCH-L3 substrate •UCH-L3 enzyme •DUB inhibitor •96-well plate |
| Applications | Useful for detecting the activity of UCH-L3 and screening the UCH-L3 inhibito/activator. |
| Size | 100 assays |
| Method | Fluorescence |
| Caution | Avoid freeze/thaw cycles |
A: UCH-L3 stands for Ubiquitin carboxyl-terminal hydrolase L3, which is a protein-coding gene.
A: UCH-L3 is a deubiquitinating enzyme that plays a role in the removal of ubiquitin, a small protein that is attached to target proteins to regulate their degradation or localization within cells.
A: UCH-L3 is expressed in various tissues and is involved in several cellular processes, including protein degradation, signal transduction, and immune response.
A: Dysregulation of UCH-L3 has been associated with certain neurodegenerative diseases, such as Parkinson's and Alzheimer's.
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