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rAAV-EF1a-fDIO(W)-EGFP-T2A-TVA

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-9

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Virus Particles Information

Cat. No. RABVH-9
Description Flp-dependent AAV helper co-expressing EGFP and TVA from a WPRE-enhanced cassette under EF1a. The WPRE element boosts transcript stability and expression, increasing both EGFP brightness in Flp-expressing starter neurons and TVA availability for entry of EnvA-pseudotyped G-deleted rabies virus, thereby improving the efficiency of Flp-based monosynaptic retrograde tracing.
Product Type Adeno-associated virus
Reporter EGFP
Expression Mechanism Flp-dependent
Helper Function AAV helper co-expressing EGFP and TVA with WPRE enhancer under Flp-dependent control
Applications
  1. Co-injection with RV-EnvA-ΔG for Flp-dependent monosynaptic retrograde tracing with enhanced expression
  2. WPRE element enhances transgene expression for stronger signal
  3. EGFP and TVA co-expressed via T2A
  4. fDIO(W) restricts expression to Flp+ populations with enhanced levels
  5. Improved starter cell visualization with high-titer helper injection
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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