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rAAV-EF1a-fDIO-oRVG

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-35

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Virus Particles Information

Cat. No. RABVH-35
Description Flp-dependent AAV helper expressing codon-optimized rabies virus glycoprotein (oRVG) under EF1a. Supplying RVG in trans complements the G deletion of EnvA-pseudotyped rabies virus, but only in Flp-expressing neurons, so transsynaptic spread is confined to the Flp-defined starter population. Used with a TVA-expressing helper, it enables Flp-specific monosynaptic retrograde tracing.
Product Type Adeno-associated virus
Expression Mechanism Flp-dependent
Helper Function AAV helper expressing codon-optimized rabies glycoprotein (oRVG) under Flp-dependent control
Applications
  1. Co-injection with TVA helper AAV and RV-EnvA-ΔG for Flp-dependent monosynaptic retrograde tracing
  2. Provides RV glycoprotein for trans-complementation of RV-ΔG
  3. oRVG (codon-optimized) for enhanced expression in mammalian neurons
  4. fDIO restricts expression to Flp+ populations
  5. Mapping direct presynaptic inputs to Flp-defined populations
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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