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rAAV-EF1a-DIO-TVA-P2A-NLS-dTomato

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-12

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Virus Particles Information

Cat. No. RABVH-12
Description Cre-dependent AAV helper co-expressing TVA and nuclear-localized dTomato via P2A under EF1a. TVA permits entry of EnvA-pseudotyped G-deleted rabies virus only into Cre-expressing neurons, and the nuclear dTomato signal gives precise, single-cell definition of these starter cells. With an RVG helper, the virus crosses one synapse retrogradely to map direct presynaptic inputs.
Product Type Adeno-associated virus
Reporter Tomato
Expression Mechanism Cre-dependent
Helper Function AAV helper expressing TVA and nuclear-localized dTomato under Cre-dependent control
Applications
  1. Co-injection with RV-EnvA-ΔG for Cre-dependent monosynaptic retrograde tracing with nuclear starter labeling
  2. NLS confines dTomato to nucleus for unambiguous starter cell identification
  3. P2A enables bicistronic expression
  4. DIO restricts expression to Cre+ populations
  5. Mapping direct presynaptic inputs to Cre-defined populations
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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