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rAAV-EF1a-DIO-RVG-P2A-mCherry-T2A-TVA

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-51

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Virus Particles Information

Cat. No. RABVH-51
Description Cre-dependent all-in-one AAV helper co-expressing RVG, mCherry and TVA from a single P2A/T2A-linked transcript under EF1a. In Cre-positive neurons, TVA permits entry of EnvA-pseudotyped G-deleted rabies virus while the co-expressed RVG complements its G deletion, enabling one retrograde transsynaptic step; mCherry marks the starter cells. This design removes the need for two separate helpers.
Product Type Adeno-associated virus
Reporter mCherry
Expression Mechanism Cre-dependent
Helper Function AAV helper co-expressing RVG, mCherry, and TVA via P2A/T2A under Cre-dependent control
Applications
  1. Co-injection with RV-EnvA-ΔG for Cre-dependent monosynaptic retrograde tracing
  2. All-in-one helper: TVA for entry + RVG for trans-complementation + mCherry for visualization
  3. P2A and T2A self-cleaving peptides enable tricistronic expression
  4. DIO restricts all components to Cre+ populations
  5. Mapping direct presynaptic inputs with single-vector simplicity
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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