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rAAV-EF1a-DIO-NLS-mCherry-F2A-TVA-T2A-RVG

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-52

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Virus Particles Information

Cat. No. RABVH-52
Description Cre-dependent all-in-one AAV helper co-expressing nuclear mCherry, TVA and RVG via F2A/T2A under EF1a. Cre-positive neurons become permissive to EnvA-pseudotyped G-deleted rabies virus through TVA, and the co-expressed RVG complements the G deletion, permitting a single retrograde transsynaptic step. Nuclear mCherry gives precise starter-cell identification from one injection.
Product Type Adeno-associated virus
Reporter mCherry
Expression Mechanism Cre-dependent
Helper Function AAV helper co-expressing nuclear mCherry, TVA, and RVG via F2A/T2A under Cre-dependent control
Applications
  1. Co-injection with RV-EnvA-ΔG for Cre-dependent monosynaptic retrograde tracing with nuclear red starter
  2. NLS-mCherry for unambiguous nuclear starter cell visualization
  3. F2A and T2A for tricistronic expression
  4. DIO restricts all components to Cre+ populations
  5. Mapping direct presynaptic inputs with unambiguous starter identification
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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