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rAAV-EF1a-DIO-N2cG-hGH

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-43

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Virus Particles Information

Cat. No. RABVH-43
Description Cre-dependent AAV helper expressing the CVS-N2c glycoprotein (N2cG) under EF1a. N2cG provides efficient, low-toxicity complementation of G-deleted rabies virus, and Cre dependence restricts glycoprotein supply, and therefore transsynaptic spread, to the targeted starter neurons. Co-injected with a TVA helper, it enables Cre-defined monosynaptic retrograde tracing.
Product Type Adeno-associated virus
Expression Mechanism Cre-dependent
Helper Function AAV helper expressing N2cG under Cre-dependent control
Applications
  1. Co-injection with TVA helper AAV and CVS-N2c-EnvA-ΔG RV for Cre-dependent monosynaptic retrograde tracing
  2. N2cG provides CVS-N2c glycoprotein for higher efficiency and lower toxicity
  3. hGH polyA for enhanced mRNA stability
  4. DIO restricts to Cre+ populations
  5. Mapping direct presynaptic inputs with reduced neurotoxicity
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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