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rAAV-EF1a-DIO-mCherry-F2A-TVA

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-18

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Virus Particles Information

Cat. No. RABVH-18
Description Cre-dependent AAV helper co-expressing mCherry and TVA via an F2A element under EF1a. The F2A configuration yields efficient co-translational separation of the two proteins, giving strong red labelling of Cre-positive starter neurons and reliable TVA expression for entry of EnvA-pseudotyped G-deleted rabies virus, thereby supporting Cre-specific retrograde monosynaptic input mapping.
Product Type Adeno-associated virus
Reporter mCherry
Expression Mechanism Cre-dependent
Helper Function AAV helper co-expressing mCherry and TVA via F2A under Cre-dependent control
Applications
  1. Co-injection with RV-EnvA-ΔG for Cre-dependent monosynaptic retrograde tracing
  2. F2A (foot-and-mouth disease virus 2A) peptide for bicistronic expression
  3. DIO restricts expression to Cre+ populations
  4. mCherry for starter cell visualization
  5. Mapping direct presynaptic inputs to Cre-defined populations
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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