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rAAV-EF1a-DIO-EGFP-T2A-TK(HSV)

For research use only. Not intended for any clinical use.

Cat. No. :   HSVH-6

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Virus Particles Information

Cat. No. HSVH-6
Description Cre-dependent AAV helper delivering HSV1 thymidine kinase (TK) and EGFP under the EF1a promoter. When co-injected with the TK-deleted HSV1-ΔTK tracer, TK is supplied only in Cre-expressing starter neurons, confining viral replication and single-synapse anterograde spread to their direct downstream targets. EGFP marks the starter population for unambiguous identification of the injection site.
Product Type Adeno-associated virus
Reporter EGFP
Expression Mechanism Cre-dependent
Helper Function AAV helper for Cre-dependent anterograde monosynaptic HSV1-ΔTK tracing with green fluorescence starter labeling
Applications
  1. Co-injection with HSV1-ΔTK-tdTomato (or HSV1-ΔTK-HBEGFP) for Cre-dependent anterograde monosynaptic tracing
  2. EF1a promoter for stable long-term broad expression
  3. EGFP reporter for visualizing helper-transduced starter cells
  4. Standard application: AAV helper injected 14–21 days before HSV1-ΔTK
  5. Mapping direct first-order outputs of Cre-defined neuronal populations
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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