rAAV-hSyn-DIO-EGFP-T2A-TK(HSV)

Name: rAAV-hSyn-DIO-EGFP-T2A-TK(HSV)
SKU: HSVH-1
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : HSVH-1

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Virus Particles Information

Cat. No.	HSVH-1
Description	Cre-dependent AAV helper delivering HSV1 thymidine kinase (TK) and EGFP under the neuron-specific hSyn promoter. Co-injected with the TK-deleted HSV1-ΔTK anterograde tracer, it supplies TK in trans so that viral replication is restricted to Cre-expressing starter neurons. EGFP labels the starter cells, while progeny virions cross a single synapse to reveal their direct downstream targets, enabling cell-type-specific anterograde monosynaptic circuit mapping.
Product Type	Adeno-associated virus
Reporter	EGFP
Expression Mechanism	Cre-dependent
Helper Function	AAV helper for Cre-dependent anterograde monosynaptic HSV1-ΔTK tracing in neurons
Applications	Co-injection with HSV1-ΔTK-tdTomato (e.g., VNTH-4) to enable Cre-dependent anterograde monosynaptic tracing of postsynaptic targets from genetically defined (Cre+) neurons AAV-TK complementation in Cre-driver mouse/rat lines for cell-type-specific HSV1-ΔTK starter cell labeling hSyn promoter restricts TK expression to neurons, minimizing off-target effects in glia EGFP reporter for visualizing helper-transduced starter cells Mapping direct first-order outputs of genetically defined neuronal populations (e.g., PV-Cre, CaMKII-Cre, DAT-Cre, GAD-Cre) Standard application: AAV helper injected 14–21 days before HSV1-ΔTK at the same brain site
Promoter	Neuron-specific Promoter
Size	Custom
Storage	Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping	Dry ice

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