Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : VNTH-6
| Cat. No. | VNTH-6 |
| Description | Cre-conditional, replication-competent anterograde polysynaptic HSV-1 H129 tracer (H356 strain design). The TK locus carries a LoxP-Stop-LoxP-flanked expression cassette linking tdTomato and a codon-modified TK (mTK) via a self-cleaving 2A peptide, all under the strong CAG promoter, with WPRE and poly(A) regulatory elements. Without Cre, the STOP blocks expression. In Cre-expressing neurons, Cre recombinase excises the STOP cassette, restoring TK expression and viral replication; the virus then propagates across multiple synapses anterogradely and labels the entire downstream circuit with tdTomato, providing cell-type-specific polysynaptic tracing. |
| Product Type | Herpes simplex virus |
| Reporter | tdTomato |
| Synaptic Transmission | Multi-synaptic (Polysynaptic, Cre-restricted) |
| TKStatus | Codon-modified TK (mTK) under Cre control (replication-competent after Cre) |
| Applications |
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| Size | Custom |
| Storage | Store at -80℃. Avoid multiple freeze/thaw cycles. |
| Shipping | Dry ice |
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