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rAAV-EF1a-DIO-mCherry-T2A-TK(HSV)

For research use only. Not intended for any clinical use.

Cat. No. :   HSVH-4

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Virus Particles Information

Cat. No. HSVH-4
Description Cre-dependent AAV helper expressing HSV1 thymidine kinase (TK) and mCherry under the EF1a promoter. Co-injection with HSV1-ΔTK limits viral replication to Cre-expressing starter neurons and restricts spread to one synapse. The red mCherry reporter allows starter cells to be identified and distinguished from green anterograde or retrograde labels, supporting multi-colour anterograde monosynaptic tracing.
Product Type Adeno-associated virus
Reporter mCherry
Expression Mechanism Cre-dependent
Helper Function AAV helper for Cre-dependent anterograde monosynaptic HSV1-ΔTK tracing with red fluorescence starter labeling
Applications
  1. Co-injection with HSV1-ΔTK-EGFP for Cre-dependent anterograde monosynaptic tracing with red starter labeling
  2. Red mCherry reporter enables spectral separation from green HSV1-ΔTK-EGFP signal
  3. EF1a promoter for stable long-term broad expression
  4. Dual-color experiments with EGFP-labeled HSV1-ΔTK
  5. Mapping direct outputs of Cre-defined neuronal populations in dual-color paradigm
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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