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rAAV-CaMKIIa-EGFP-T2A-TK(HSV)

For research use only. Not intended for any clinical use.

Cat. No. :   HSVH-10

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Virus Particles Information

Cat. No. HSVH-10
Description Constitutive AAV helper expressing HSV1 thymidine kinase (TK) and EGFP under the CaMKIIa promoter, which restricts expression to excitatory neurons. Co-injected with HSV1-ΔTK, it complements the deleted TK gene and permits single-synapse anterograde spread from excitatory starter neurons, allowing the downstream targets of cortical and hippocampal pyramidal cells to be mapped without any recombinase requirement.
Product Type Adeno-associated virus
Reporter EGFP
Helper Function AAV helper for constitutive anterograde monosynaptic HSV1-ΔTK tracing specifically in excitatory neurons
Applications
  1. Co-injection with HSV1-ΔTK-tdTomato for constitutive anterograde monosynaptic tracing in excitatory pyramidal neurons
  2. CaMKIIα promoter restricts TK expression to excitatory glutamatergic neurons (forebrain pyramidal cells)
  3. EGFP reporter for visualizing starter cells
  4. No Cre/Flp requirement — useful when cell-type-specific targeting is achieved by promoter alone
  5. Mapping direct postsynaptic targets of excitatory projection neurons
  6. Standard application in cortical, hippocampal, and amygdalar circuits
Promoter Neuron-specific Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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