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rAAV-EF1a-Con/Fon-H2B-EGFP-P2A-TVA

For research use only. Not intended for any clinical use.

Cat. No. :   RABVH-23

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Virus Particles Information

Cat. No. RABVH-23
Description AAV helper using a Con/Fon (Cre-ON AND Flp-ON) Boolean cassette to drive nuclear H2B-EGFP and TVA via P2A. TVA is expressed only in neurons that contain both Cre and Flp, so EnvA-pseudotyped G-deleted rabies virus infects an intersectional starter population. With RVG, this enables dual-recombinase-defined monosynaptic retrograde input mapping.
Product Type Adeno-associated virus
Reporter EGFP
Expression Mechanism Boolean Algebra
Helper Function AAV helper for Cre AND Flp intersectional TVA expression (Boolean AND gate)
Applications
  1. Co-injection with RV-EnvA-ΔG for Cre AND Flp intersectional monosynaptic retrograde tracing
  2. Con/Fon expresses TVA only when BOTH Cre and Flp are present
  3. Nuclear-localized EGFP reporter for starter cell visualization
  4. Enables targeting of cells defined by two intersecting genetic markers
  5. Mapping direct presynaptic inputs to intersectional populations
  6. Powerful for targeting projection-defined or state-dependent subpopulations
Promoter Broad-spectrum Promoter
Size Custom
Storage Store at -80℃. Avoid multiple freeze/thaw cycles.
Shipping Dry ice
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