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Mouse Tnfrsf17 Stable Cell Line - MC38

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO02526

Host Cell :   MC38 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RO02526
Description This cell line is engineered to stably overexpress Mouse Tnfrsf17 in MC38 cell line. GFP reporter gene is also expressed in this cell line for cell tracking.
Product Type Stable cell line constitutively expressing target gene
Target Gene Tnfrsf17
Gene Species Mouse
Host Cell MC38
Host Cell Species Mus musculus (Mouse)
Applications Gene function studies and others
Size One vial of frozen cells, typically >1x10^6cells/vial
Stability This cell line is stable at least 10 passages.
Quality Control 1) Detection of gene overexpression by Real-time qPCR ,or western blot, or flow cytometry,
2) Mycoplasma detection
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Adherent
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Target Gene TNFRSF17
Background The protein encoded by this gene is a member of the TNF-receptor superfamily. This receptor is preferentially expressed in mature B lymphocytes, and may be important for B cell development and autoimmune response. This receptor has been shown to specifically bind to the tumor necrosis factor (ligand) superfamily, member 13b (TNFSF13B/TALL-1/BAFF), and to lead to NF-kappaB and MAPK8/JNK activation. This receptor also binds to various TRAF family members, and thus may transduce signals for cell survival and proliferation. [provided by RefSeq, Jul 2008]
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