Cat.No. : CSC-RO1057 Host Cell: HEK293T
| Cat. No. | CSC-RO1057 |
| Description | HEK293-TNFRSF17 cell line is engineered to stably overexpress human TNFRSF17. |
| Gene | TNFRSF17 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human TNFRSF17 Stable Cell Line-HEK293 originates from human embryonic kidney 293 (HEK293) cells that were transfected with a stable expression construct containing the human TNFRSF17 gene. This gene encodes for the tumor necrosis factor receptor superfamily member 17, also known as BCMA (B-cell maturation antigen). The cell line was established through a transfection and selection process to create stable cell populations expressing human TNFRSF17.
A: The Human TNFRSF17 Stable Cell Line-HEK293 is primarily used in research related to B-cell biology, immunology, and cancer. It is valuable for studying the interaction between BCMA and its ligand APRIL (a proliferation-inducing ligand) and exploring the signaling pathways involved in B-cell maturation and survival. Researchers also use this cell line for drug discovery and development, especially in the context of multiple myeloma therapies targeting BCMA.
A: The interaction between BCMA and APRIL in the Human TNFRSF17 Stable Cell Line-HEK293 typically activates signaling pathways such as NF-κB and JNK (c-Jun N-terminal kinase). These pathways are essential for B-cell survival and proliferation. Additionally, the activation of BCMA by APRIL can lead to the upregulation of anti-apoptotic proteins, contributing to B-cell survival.
A: One potential challenge is that the Human TNFRSF17 Stable Cell Line-HEK293 represents a simplified model of BCMA signaling, and its behavior may not fully mimic the complexity of in vivo B-cell interactions. Researchers should also consider that the stability of the cell line and the consistency of BCMA expression can be critical for experimental reproducibility. Careful validation and characterization of the cell line are important.
A: To maintain the Human TNFRSF17 Stable Cell Line-HEK293, it is typically cultured in a suitable growth medium containing high-glucose DMEM (Dulbecco's Modified Eagle Medium) supplemented with Fetal Bovine Serum (FBS) and antibiotics such as penicillin and streptomycin. The exact conditions may vary depending on the specific research goals, but maintaining a stable temperature (37°C) and CO2 concentration (5%) is standard.
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