Human TNFRSF17 Stable Cell Line-HEK293T

Name: Human TNFRSF17 Stable Cell Line-HEK293T
SKU: CSC-RO1057
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1057

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO1057
Description	HEK293-TNFRSF17 cell line is engineered to stably overexpress human TNFRSF17.
Target Gene	TNFRSF17
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	TNFRSF17 tumor necrosis factor receptor superfamily, member 17 [ Homo sapiens ]
Gene Symbol	TNFRSF17
Synonyms	BCM; BCMA; CD269
Gene Description	tumor necrosis factor receptor superfamily, member 17
GeneID	608
Uni ProtID	Q02223
mRNA Refseq	NM_001192.2
Protein Refseq	NP_001183.2
Chromosome Location	16p13.1
Function	receptor activity; tumor necrosis factor-activated receptor activity;
Pathway	Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Intestinal immune network for IgA production, organism-specific biosystem; Intestinal immune network for IgA production, conserved biosystem;
MIM	109545

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Q & A

Customer Reviews

Customer Q&As

What is the specific origin of the Human TNFRSF17 Stable Cell Line-HEK293, and how was it established?

A: The Human TNFRSF17 Stable Cell Line-HEK293 originates from human embryonic kidney 293 (HEK293) cells that were transfected with a stable expression construct containing the human TNFRSF17 gene. This gene encodes for the tumor necrosis factor receptor superfamily member 17, also known as BCMA (B-cell maturation antigen). The cell line was established through a transfection and selection process to create stable cell populations expressing human TNFRSF17.

What are the primary applications of the Human TNFRSF17 Stable Cell Line-HEK293 in research or biotechnology?

A: The Human TNFRSF17 Stable Cell Line-HEK293 is primarily used in research related to B-cell biology, immunology, and cancer. It is valuable for studying the interaction between BCMA and its ligand APRIL (a proliferation-inducing ligand) and exploring the signaling pathways involved in B-cell maturation and survival. Researchers also use this cell line for drug discovery and development, especially in the context of multiple myeloma therapies targeting BCMA.

Can you provide insights into the signaling pathways activated by the interaction of BCMA with APRIL in the Human TNFRSF17 Stable Cell Line-HEK293?

A: The interaction between BCMA and APRIL in the Human TNFRSF17 Stable Cell Line-HEK293 typically activates signaling pathways such as NF-κB and JNK (c-Jun N-terminal kinase). These pathways are essential for B-cell survival and proliferation. Additionally, the activation of BCMA by APRIL can lead to the upregulation of anti-apoptotic proteins, contributing to B-cell survival.

Are there any known challenges or limitations associated with working with the Human TNFRSF17 Stable Cell Line-HEK293 that researchers should be aware of?

A: One potential challenge is that the Human TNFRSF17 Stable Cell Line-HEK293 represents a simplified model of BCMA signaling, and its behavior may not fully mimic the complexity of in vivo B-cell interactions. Researchers should also consider that the stability of the cell line and the consistency of BCMA expression can be critical for experimental reproducibility. Careful validation and characterization of the cell line are important.

What specific experimental conditions and media are recommended for maintaining the Human TNFRSF17 Stable Cell Line-HEK293?

A: To maintain the Human TNFRSF17 Stable Cell Line-HEK293, it is typically cultured in a suitable growth medium containing high-glucose DMEM (Dulbecco's Modified Eagle Medium) supplemented with Fetal Bovine Serum (FBS) and antibiotics such as penicillin and streptomycin. The exact conditions may vary depending on the specific research goals, but maintaining a stable temperature (37°C) and CO2 concentration (5%) is standard.

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