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Human TNFRSF17 Stable Cell Line-HEK293T

Human TNFRSF17 Stable Cell Line-HEK293T

Cat.No. :  CSC-RO0095 Host Cell:  HEK293T

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Cell Line Information

Cell Culture Information

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Cat. No. CSC-RO0095
Description HEK293T-TNFRSF17 cell line is a stably transfected cell line which expresses human TNF receptor superfamily member 17 (TNFRSF17).
Gene TNFRSF17
Gene Species Homo sapiens (Human)
Host Cell HEK293T
Host Cell Species Homo sapiens (Human)
Stability Validated for at least 10 passages
Application

1. Studying the interactions between immune cells and cancer cells

2. Studying the mechanisms of resistance to immune checkpoint blockade

3. High-throughput screening

4. Drug target validation

Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Shipping Dry ice
Storage Liquid nitrogen
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations

The following safety precautions should be observed.

1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.

2. No eating, drinking or smoking while handling the stable line.

3. Wash hands after handling the stable line and before leaving the lab.

4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.

5. All waste should be considered hazardous.

6. Dispose of all liquid waste after each experiment and treat with bleach.

Ship Dry ice
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