Human MC2R Stable Cell Line - HEK293

Name: Human MC2R Stable Cell Line - HEK293
SKU: CSC-RG01853

Cat.No. : CSC-RG01853 Host Cell: HEK293

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Cat. No.	CSC-RG01853
Description	This cell line is engineered to stably overexpress human MC2R in HEK293 cells.
Gene	MC2R
Gene Species	Homo sapiens (Human)
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Stability	This cell line is stable at least 10 passages.
Product Type	Stable cell line constitutively expressing human kinase gene(s)
Applications	HEK293 stable cell lines expressing GPCR genes have a wide range of uses in basic research and drug discovery. 1) High - throughput Screening: These cell lines are widely used in high - throughput screening assays to identify new ligands for GPCRs. By exposing the cells to large libraries of small molecules or other compounds and monitoring the resulting cellular responses, potential drug candidates can be rapidly identified. 2) Drug Target Validation: They provide a valuable tool for validating GPCRs as drug targets. Researchers can study the effects of specific drugs or genetic manipulations on the GPCR - expressing cells to assess the functional significance of the GPCR in a particular biological process. This helps to determine whether targeting a specific GPCR is likely to have therapeutic benefits. 3) Pharmacological Characterization: HEK293 stable cell lines allow for the detailed pharmacological characterization of GPCR ligands. Parameters such as binding affinity, efficacy, and potency can be determined by measuring the ligand - induced responses in these cells. This information is crucial for optimizing the design of new drugs and understanding their mechanism of action.
Quality Control	1) Real-time qPCR analysis of gene mRNA overexpression level 2) Analysis of GPCR function by cAMP assay 3) mycoplasma detection
Size Form	One vial of frozen cells, typically >1x10^6cells/vial
Shipping	Dry ice
Storage	Liquid nitrogen

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm² flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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