Human HCAR3 Stable Cell Line-CHO-K1

Name: Human HCAR3 Stable Cell Line-CHO-K1
SKU: CSC-RG0561
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG0561

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG0561
Description	This cell line is engineered to stably overexpress human HCAR3 in CHO-K1.
Target Gene	HCAR3
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Size	>1x10⁶ frozen cells/vial
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	HCAR3 hydroxycarboxylic acid receptor 3 [ Homo sapiens ]
Gene Symbol	HCAR3
Synonyms	HCA3; HM74; PUMAG; Puma-g; GPR109B
Gene ID	8843
Uni Prot ID	E9PI97
m RNA Refseq	NM_006018.2
Protein Refseq	NP_006009.2
Chromosome Location	12q24.31
Function	G-protein coupled purinergic nucleotide receptor activity; G-protein coupled receptor activity;
Pathway	Class A/1 (Rhodopsin-like receptors), organism-specific biosystem; G alpha (i) signalling events, organism-specific biosystem; GPCR downstream signaling, organism-specific biosystem; GPCR ligand binding, organism-specific biosystem; GPCRs, Class A Rhodopsin-like, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by GPCR, organism-specific biosystem;
MIM	606039

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