Human GPR174 Stable Cell Line - HEK293

GPR174, officially designated as G protein-coupled receptor 174, is a member of the rhodopsin-like GPCR superfamily characterized by seven transmembrane domains. The gene is located on the X chromosome at Xq21.1 and encodes a protein of approximately 333 amino acids. Initially classified as an orphan receptor, GPR174 has since been identified as a receptor for lysophosphatidylserine (LysoPS), a bioactive lipid involved in immune regulation. It is also referred to as LPS3, LYPSR3, or FKSG79. The Human GPR174 Stable Cell Line - HEK293 is generated by transfecting the full-length human GPR174 coding sequence into HEK293 cells, followed by antibiotic selection to establish a population that stably expresses the receptor on the plasma membrane. This system provides a reliable and reproducible in vitro platform for studying GPR174 function, as the receptor is expressed at consistent levels across passages, enabling robust and repeatable experimental outcomes.

This stable cell line is widely used in a range of research applications, including ligand binding assays, second messenger detection, and GPCR signaling pathway characterization. GPR174 is known to couple primarily to Gαs proteins, leading to increased intracellular cAMP levels upon activation. Researchers can use this cell line to evaluate the potency and efficacy of candidate ligands, including synthetic LysoPS analogs or other potential agonists and antagonists. It is also a valuable tool for investigating the receptor’s role in immune cell modulation, as GPR174 has been implicated in the regulation of T cell and dendritic cell functions, as well as in autoimmune conditions such as Graves’ disease and Addison’s disease. Additionally, the cell line supports high-throughput screening efforts aimed at identifying novel small molecule modulators, making it suitable for both basic research and early-stage drug discovery programs targeting GPR174.