Human CALCRL/RAMP1 Stable Cell Line - HEK293

The calcitonin receptor-like receptor (CALCRL) and receptor activity regulator 1 (RAMP1) are key components of the calcitonin gene-related peptide (CGRP) receptor complex. CALCRL is a G protein-coupled receptor (GPCR) whose functional expression and ligand specificity depend on RAMP1. RAMP1 is a single-pass transmembrane protein responsible for transporting CALCRL to the cell surface and determining its ligand selectivity. CALCRL and RAMP1 together form a heterodimeric receptor that binds to CGRP. CGRP is a potent neuropeptide involved in the pathophysiology of vasodilation, neurogenic inflammation, and migraine. The CALCRL/RAMP1 complex plays a crucial role in cardiovascular regulation, nociception, and inflammatory responses, making it an important therapeutic target for migraine and cardiovascular diseases. Genetic variations in CALCRL or RAMP1 are associated with vascular diseases, highlighting their significant physiological importance.

The human CALCRL/RAMP1 stable cell line HEK293 is an engineered cell model specifically designed for high-throughput screening and pharmacological studies of CGRP receptor modulators. Constructed by transfecting the CALCRL and RAMP1 genes into human embryonic kidney cells (HEK293), this cell line stably co-expresses both proteins, ensuring congruence of receptor function. HEK293 cells were chosen for their high transfection efficiency, robust growth, and extremely low levels of endogenous GPCR expression, providing a pristine background for receptor studies. This stable cell line can be used for ligand binding assays, calcium ion flux measurements, cAMP detection, and receptor internalization studies, enabling precise evaluation of agonists, antagonists, and allosteric modulators. Its reliability makes it invaluable in drug discovery for migraine treatments, such as CGRP antagonists, accelerating preclinical research and development.