ODN 1826

Name: ODN 1826
SKU: CGON-08
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CGON-08

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Product Information

Cat. No.	CGON-08
Description	CpG oligodeoxynucleotides (or CpG ODNs) are synthetic oligonucleotides that contain unmethylated CpG motifs. CpG ODN can bind to and activate a Toll-like receptor 9 (TLR9) and leading to strong immunostimulatory effects. So far 3 major classes of CpG ODNs have been identified, based on their structural and biological characteristics, and are designated Type A, Type B, and Type C. Type A ODNs, which feature a central palindromic CpG-containing phosphodiester (PO) structure followed by a phosphorothioate (PS) homopolymeric G-stretch, are robust inducers of interferon-α (IFN-α) production and dendritic cell maturation. Type B ODNs, in contrast, contain a full phosphorothioate backbone with one or more CpG dinucleotides. They strongly activate B cells but stimulate weakly IFN-α secretion. Type C ODNs, combine the properties of both Type A and B, and are characterized by their complete PS backbone and palindromic CpG-containing motifs. Type C ODNs induce strong IFN-α production from pDC and B cell stimulation. ODN 1826 is a type B CpG ODN specific for murine TLR9.
Features	• Classification: Type B • Product format: Lyophilized product • Sequence: 5'-tccatgacgttcctgacgtt-3' (lower case letters are phosphorothioate) • Specificity: Mouse, Rat
Storage	Store lyophilized product at -20°C. Upon reconstitution, aliquots should be stored at -20°C and are stable for 6 months. Avoid repeated freeze-thaw cycles.

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What is the primary biological mechanism of ODN 1826?

A: ODN 1826 is a CpG oligonucleotide that enhances immune response by activating Toll-like receptor 9 (TLR9), playing a role in tumor immunotherapy and vaccine adjuvant settings.

How to determine the optimal concentration and duration of action for ODN 1826 in tumor immunotherapy research?

A: Determine the optimal concentration through dose-response experiments, considering the specificity of cell types and tumor models, and identify the most effective duration of action through time-course experiments.

How to correctly add and handle ODN 1826 in cell culture?

A: Dilute ODN 1826 to an appropriate concentration according to the experimental design and add it evenly to the culture medium. Ensure sterile conditions to avoid cell contamination.

How to assess the effect of ODN 1826 on activating immune cells in research?

A: Detect changes in the expression of surface markers on immune cells using flow cytometry, or measure the secretion levels of cytokines using techniques like ELISA to evaluate its immunostimulatory effects.

How to assess potential cytotoxicity when using ODN 1826?

A: Evaluate the impact of different concentrations of ODN 1826 on cell viability using assays like MTT or WST-1 to identify potential cytotoxic effects.

How stable is ODN 1826 during long-term storage, and how should it be properly stored?

A: Store ODN 1826 in dry, light-protected, and cold conditions, avoiding repeated freeze-thaw cycles to maintain its stability and biological activity.

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