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Cat.No. : CSC-RO0863 Host Cell: HEK293T
| Cat. No. | CSC-RO0863 |
| Description | This cell line is engineered to stably overexpress mouse ICOSL. |
| Gene | Icosl |
| Gene Species | Mus musculus (Mouse) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Mouse Icosl Stable Cell Line - HEK293T is typically derived from HEK293T cells by stable transfection of the Icosl gene. Its stability is ensured through continuous culturing and monitoring of Icosl gene expression.
A: In studies of immune co-stimulatory signals, Mouse Icosl Stable Cell Line - HEK293T can simulate T cell activation by expressing Icosl, thereby allowing the investigation of the signaling mechanisms involved in T cell co-stimulation.
A: In tumor immunotherapy research, Mouse Icosl Stable Cell Line - HEK293T can be used to evaluate the efficacy of immunotherapeutic strategies, such as Icosl antibody treatment, and to study its role in enhancing T cell immune responses.
A: The activity of the ASIC3 channel is typically evaluated in Mouse Icosl Stable Cell Line - HEK293T through electrophysiological recordings of channel activity and calcium ion imaging, among other techniques. The effects of drugs can be assessed using these experiments.
A: Addressing potential cell toxicity or adaptation issues may require optimizing culture conditions, screening for appropriate culture media, and adding relevant cytokines to ensure the health and adaptability of the cells in research.
A: Innovative applications of Mouse Icosl Stable Cell Line - HEK293T in immune co-stimulation research include studying novel co-stimulatory molecules, exploring new mechanisms of immune therapy, and evaluating new drugs for immunotherapy.
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