Mouse Icosl Stable Cell Line - HEK293T

Name: Mouse Icosl Stable Cell Line - HEK293T
SKU: CSC-RO0863
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0863

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0863
Description	This cell line is engineered to stably overexpress mouse ICOSL.
Target Gene	Icosl
Gene Species	Mus musculus (Mouse)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Icosl icos ligand [ Mus musculus ]
Gene Symbol	Icosl
Synonyms	B7h; GI50; GL50; B7-H2; LICOS; B7RP-1; GL50-B; ICOS-L; Icoslg; Ly115l; AU044799; BG071784; mKIAA0653
Gene Description	icos ligand
Gene ID	50723
Uni Prot ID	Q544C7
m RNA Refseq	NM_015790.3
Protein Refseq	NP_056605.1
Chromosome Location	10 C1; 10
Function	protein binding; receptor binding;
Pathway	Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Costimulation by the CD28 family, organism-specific biosystem; Immune System, organism-specific biosystem; Intestinal immune network for IgA production, organism-specific biosystem; Intestinal immune network for IgA production, conserved biosystem;

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Q & A

Customer Reviews

Customer Q&As

What is the source of the Mouse Icosl Stable Cell Line - HEK293T, and how is its stability ensured?

A: The Mouse Icosl Stable Cell Line - HEK293T is typically derived from HEK293T cells by stable transfection of the Icosl gene. Its stability is ensured through continuous culturing and monitoring of Icosl gene expression.

How does the Mouse Icosl Stable Cell Line - HEK293T simulate T cell activation in studies of immune co-stimulatory signals?

A: In studies of immune co-stimulatory signals, Mouse Icosl Stable Cell Line - HEK293T can simulate T cell activation by expressing Icosl, thereby allowing the investigation of the signaling mechanisms involved in T cell co-stimulation.

What are some specific applications of Mouse Icosl Stable Cell Line - HEK293T in tumor immunotherapy research?

A: In tumor immunotherapy research, Mouse Icosl Stable Cell Line - HEK293T can be used to evaluate the efficacy of immunotherapeutic strategies, such as Icosl antibody treatment, and to study its role in enhancing T cell immune responses.

How is the activity of the ASIC3 channel evaluated in Mouse Icosl Stable Cell Line - HEK293T for drug screening?

A: The activity of the ASIC3 channel is typically evaluated in Mouse Icosl Stable Cell Line - HEK293T through electrophysiological recordings of channel activity and calcium ion imaging, among other techniques. The effects of drugs can be assessed using these experiments.

How is potential cell toxicity or adaptation issues addressed when using Mouse Icosl Stable Cell Line - HEK293T in research?

A: Addressing potential cell toxicity or adaptation issues may require optimizing culture conditions, screening for appropriate culture media, and adding relevant cytokines to ensure the health and adaptability of the cells in research.

What are some innovative applications of Mouse Icosl Stable Cell Line - HEK293T in immune co-stimulation research?

A: Innovative applications of Mouse Icosl Stable Cell Line - HEK293T in immune co-stimulation research include studying novel co-stimulatory molecules, exploring new mechanisms of immune therapy, and evaluating new drugs for immunotherapy.

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