Human PVR Stable Cell Line - HEK293T

Name: Human PVR Stable Cell Line - HEK293T
SKU: CSC-RO0551
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0551

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

Inquire for Price

Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0551
Description	This cell line is derived from HEK293T and is engineered to stably overexpress Human PVR.
Target Gene	PVR
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	PVR poliovirus receptor [ Homo sapiens ]
Gene Symbol	PVR
Synonyms	PVR; poliovirus receptor; PVS; CD155; HVED; Necl 5; NECL5; nectin like 5; Tage4; nectin-like 5; nectin-like protein 5; TAGE4; Necl-5; FLJ25946;
GeneID	5817
Uni ProtID	P15151
mRNA Refseq	BC015542
Chromosome Location	19q13.2
Function	cell adhesion molecule binding; protein binding; receptor activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Adherens junctions interactions, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell junction organization, organism-specific biosystem; Cell-Cell communication, organism-specific biosystem; Cell-cell junction organization, organism-specific biosystem;
MIM	173850

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What is the key role of the Human PVR Stable Cell Line - HEK293T in studying the interaction between PVR and viruses?

A: The key role of the Human PVR Stable Cell Line - HEK293T in studying the interaction between PVR (Poliovirus Receptor) and viruses lies in providing a stable cell model for investigating how PVR acts as a viral receptor in the infection process. This cell line allows researchers to simulate the interaction between viruses and host cells, particularly in the process of PVR-mediated virus attachment and endocytosis, which is crucial for understanding viral entry mechanisms and developing antiviral strategies.

How can the Human PVR Stable Cell Line - HEK293T cell line be utilized for PVR gene knockout studies?

A: The Human PVR Stable Cell Line - HEK293T cell line can be utilized for PVR gene knockout studies by employing CRISPR/Cas9 technology to precisely introduce mutations in the PVR gene, thereby creating PVR gene knockout cell lines. These knockout cell lines can be used to study the role of PVR in cellular functions and immune responses, as well as its importance in viral infections.

What is the role of the Human PVR Stable Cell Line - HEK293T cell line in studying the role of PVR in tumor cell migration and invasion?

A: The role of the Human PVR Stable Cell Line - HEK293T cell line in studying the role of PVR in tumor cell migration and invasion lies in providing a platform for exploring how PVR influences the behavior of tumor cells. Through this cell line, researchers can observe how changes in PVR expression levels affect the migratory capacity and invasiveness of tumor cells, which is significant for understanding tumor metastasis mechanisms and developing new therapeutic strategies.

What is the role of the Human PVR Stable Cell Line - HEK293T cell line in PVR-mediated immune cell adhesion and activation?

A: The role of the Human PVR Stable Cell Line - HEK293T cell line in PVR-mediated immune cell adhesion and activation lies in providing a model system for studying how PVR acts as an immune cell receptor in the modulation of immune cell functions. This cell line can help researchers understand the role of PVR in the regulation of immune cell functions, particularly in the adhesion and effector functions of natural killer (NK) cells.

How can the Human PVR Stable Cell Line - HEK293T cell line be used for PVR protein expression and purification studies?

A: The Human PVR Stable Cell Line - HEK293T cell line can be used for PVR protein expression and purification studies by transfecting the cells with expression vectors containing the PVR gene, leveraging the cell's high expression capabilities to produce PVR protein. Subsequently, PVR protein can be purified through a series of biochemical methods, such as affinity chromatography and ion exchange chromatography, for structural and functional studies.

What is the potential application of the Human PVR Stable Cell Line - HEK293T cell line in studying the role of PVR in tumor immunotherapy?

A: The potential application of the Human PVR Stable Cell Line - HEK293T cell line in studying the role of PVR in tumor immunotherapy lies in assessing the potential of PVR as a target for immunotherapy. Through this cell line, researchers can simulate the expression pattern of PVR on tumor cells and how it affects the recognition and attack of tumor cells by immune cells, which is valuable for developing new tumor immunotherapy strategies.

Ask a Question

If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.

Question *

Name

Country *

Email *

Write a Review

Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.

Product Name *

Catalog Name *

Rating

Needs improvement

Satisfaction

General satisfaction

Very satisfaction

Product Review Title *

Summary *