Cat.No. : CSC-RO0551 Host Cell: HEK293T
| Cat. No. | CSC-RO0551 |
| Description | This cell line is derived from HEK293T and is engineered to stably overexpress Human PVR. |
| Gene | PVR |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The key role of the Human PVR Stable Cell Line - HEK293T in studying the interaction between PVR (Poliovirus Receptor) and viruses lies in providing a stable cell model for investigating how PVR acts as a viral receptor in the infection process. This cell line allows researchers to simulate the interaction between viruses and host cells, particularly in the process of PVR-mediated virus attachment and endocytosis, which is crucial for understanding viral entry mechanisms and developing antiviral strategies.
A: The Human PVR Stable Cell Line - HEK293T cell line can be utilized for PVR gene knockout studies by employing CRISPR/Cas9 technology to precisely introduce mutations in the PVR gene, thereby creating PVR gene knockout cell lines. These knockout cell lines can be used to study the role of PVR in cellular functions and immune responses, as well as its importance in viral infections.
A: The role of the Human PVR Stable Cell Line - HEK293T cell line in studying the role of PVR in tumor cell migration and invasion lies in providing a platform for exploring how PVR influences the behavior of tumor cells. Through this cell line, researchers can observe how changes in PVR expression levels affect the migratory capacity and invasiveness of tumor cells, which is significant for understanding tumor metastasis mechanisms and developing new therapeutic strategies.
A: The role of the Human PVR Stable Cell Line - HEK293T cell line in PVR-mediated immune cell adhesion and activation lies in providing a model system for studying how PVR acts as an immune cell receptor in the modulation of immune cell functions. This cell line can help researchers understand the role of PVR in the regulation of immune cell functions, particularly in the adhesion and effector functions of natural killer (NK) cells.
A: The Human PVR Stable Cell Line - HEK293T cell line can be used for PVR protein expression and purification studies by transfecting the cells with expression vectors containing the PVR gene, leveraging the cell's high expression capabilities to produce PVR protein. Subsequently, PVR protein can be purified through a series of biochemical methods, such as affinity chromatography and ion exchange chromatography, for structural and functional studies.
A: The potential application of the Human PVR Stable Cell Line - HEK293T cell line in studying the role of PVR in tumor immunotherapy lies in assessing the potential of PVR as a target for immunotherapy. Through this cell line, researchers can simulate the expression pattern of PVR on tumor cells and how it affects the recognition and attack of tumor cells by immune cells, which is valuable for developing new tumor immunotherapy strategies.
If your question is not addressed through these resources, you can fill out the online form below and we will answer your question as soon as possible.
Write a review of your use of Biogene products and services in your research. Your review can help your fellow researchers make informed purchasing decisions.
Our promise to you:
Guaranteed product quality, expert customer support.
24x7 CUSTOMER SERVICE
CONTACT US TO ORDER
Copyright © Creative Biogene. All rights reserved.
