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Human PVR Stable Cell Line - HEK293

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO01151

Host Cell :   HEK293 Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

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Gene Information

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Cat. No. CSC-RO01151
Description This cell line is engineered to stably overexpress human PVR cell adhesion molecule (PVR) in HEK293 cells.
Target Gene PVR
Gene Species Homo sapiens (Human)
Host Cell HEK293
Host Cell Species Homo sapiens (Human)
Applications

1. Studying the interactions between immune cells and cancer cells

2. Studying the mechanisms of resistance to immune checkpoint blockade

3. High-throughput screening

4. Drug target validation

Size >1x106 frozen cells/vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Media Type Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Freeze Medium Complete medium supplemented with 10% (v/v) DMSO
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name PVR
Gene Symbol PVR
GeneID 5817
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Excellent product

The Human PVR (CD155) stable line in HEK293 cells provides consistent, high-level expression of the poliovirus receptor. We have used it extensively in TIGIT/PVR binding assays and cell-based functional studies. The line is easy to thaw and culture, and the results are always reproducible.

France

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