Human PVR Stable Cell Line - HEK293

The poliovirus receptor (PVR), also known as CD155, is a transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is encoded by the PVR gene located on human chromosome 19q13.31 and is specific to the primate lineage. PVR functions as an adhesion molecule that mediates cell attachment to the extracellular matrix component vitronectin through its external domain, while its intracellular domain interacts with the dynein light chain Tctex-1/DYNLT1. It is best known as the cellular receptor for poliovirus, facilitating the first step of viral replication. The Human PVR Stable Cell Line - HEK293 is generated by stably transfecting the human PVR gene into HEK293 cells, a widely used human embryonic kidney cell line known for its high transfectability and robust protein expression.

This stable cell line serves as a valuable tool for a wide range of research applications. It is particularly useful for studying immune checkpoint interactions involving PVR and its binding partners on immune cells, including DNAM-1 (CD226), TIGIT, and CD96, which play critical roles in natural killer cell and T cell mediated cytotoxicity. Researchers use this cell line to evaluate the efficacy of therapeutic antibodies, bispecific molecules, or small molecule inhibitors designed to block the PVR-TIGIT or PVR-DNAM-1 pathways in cancer immunotherapy. It also supports functional assays for investigating PVR-mediated cell adhesion, viral entry mechanisms, and HIV-1 Nef/Vpu-mediated downregulation of surface PVR. Additionally, the line can be employed for antibody screening, binding affinity studies, and as a positive control in flow cytometry and immunohistochemistry experiments. The stable expression ensures minimal batch-to-batch variation, making it reliable for high-throughput screening and drug development programs targeting the PVR/CD155 immune checkpoint axis.