Cat.No. : CSC-RO0511 Host Cell: CHO-K1
| Cat. No. | CSC-RO0511 |
| Description | This cell line is derived from CHO-K1 and is engineered to stably overexpress membrane OKT3 (anti-CD3 monoclonal antibody) scFV sequence. |
| Gene | OKT3 scFv |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-K1 |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human OKT3 scFv Stable Cell Line - CHO-K1, with its stable expression of the single-chain variable fragment (scFv) antibody OKT3, has significant potential for immunotherapy research. OKT3 targets the CD3 complex and can be used to study T-cell mediated immune responses, as well as to develop therapeutic strategies targeting specific immune cell subgroups.
A: The Human OKT3 scFv Stable Cell Line - CHO-K1 can be utilized in co-culture experiments to study the mechanisms of T-cell activation and regulation. By monitoring changes in the expression of T-cell surface markers and the release of cytokines, the pathways of T-cell activation mediated by OKT3 scFv can be deeply understood.
A: For drug screening, experiments should be designed to evaluate the effects of different compounds on the expression and function of the Human OKT3 scFv Stable Cell Line - CHO-K1. Potential immunomodulators can be screened by measuring the levels of OKT3 scFv expression on the cell surface and biological markers of T-cell activation.
A: The Human OKT3 scFv Stable Cell Line - CHO-K1 can be used to study immune tolerance and immune escape mechanisms. By simulating interactions between T-cells and tumor cells or other immune cells, the mechanisms of immune escape and the establishment of tolerance states can be explored.
A: When conducting gene editing with Human OKT3 scFv Stable Cell Line - CHO-K1, it is important to maintain the expression and function of OKT3 scFv. Additionally, the choice and design of gene editing tools, such as CRISPR/Cas9, should ensure specificity to the target gene and accurate validation of the editing effects.
A: The Human OKT3 scFv Stable Cell Line - CHO-K1 has promising applications in studying cell communication, especially the interactions between immune cells. This cell line can be used to investigate the interactions between T-cell receptors and antigen-presenting cells, providing an important tool for understanding intercellular communication in immune responses.
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