Human OKT3 scFv Stable Cell Line - CHO-K1

Name: Human OKT3 scFv Stable Cell Line - CHO-K1
SKU: CSC-RO0511
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0511

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Cat. No.	CSC-RO0511
Description	This cell line is derived from CHO-K1 and is engineered to stably overexpress membrane OKT3 (anti-CD3 monoclonal antibody) scFV sequence.
Target Gene	OKT3 scFv
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

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Q & A

Customer Reviews

Customer Q&As

What is the potential application of the Human OKT3 scFv Stable Cell Line - CHO-K1 in immunotherapy research?

A: The Human OKT3 scFv Stable Cell Line - CHO-K1, with its stable expression of the single-chain variable fragment (scFv) antibody OKT3, has significant potential for immunotherapy research. OKT3 targets the CD3 complex and can be used to study T-cell mediated immune responses, as well as to develop therapeutic strategies targeting specific immune cell subgroups.

How can the Human OKT3 scFv Stable Cell Line - CHO-K1 be used to study the mechanisms of T-cell activation and regulation?

A: The Human OKT3 scFv Stable Cell Line - CHO-K1 can be utilized in co-culture experiments to study the mechanisms of T-cell activation and regulation. By monitoring changes in the expression of T-cell surface markers and the release of cytokines, the pathways of T-cell activation mediated by OKT3 scFv can be deeply understood.

How should experiments be designed for drug screening using Human OKT3 scFv Stable Cell Line - CHO-K1?

A: For drug screening, experiments should be designed to evaluate the effects of different compounds on the expression and function of the Human OKT3 scFv Stable Cell Line - CHO-K1. Potential immunomodulators can be screened by measuring the levels of OKT3 scFv expression on the cell surface and biological markers of T-cell activation.

What is the application of the Human OKT3 scFv Stable Cell Line - CHO-K1 in studying immune tolerance and immune escape mechanisms?

A: The Human OKT3 scFv Stable Cell Line - CHO-K1 can be used to study immune tolerance and immune escape mechanisms. By simulating interactions between T-cells and tumor cells or other immune cells, the mechanisms of immune escape and the establishment of tolerance states can be explored.

What factors should be considered when conducting gene editing experiments with Human OKT3 scFv Stable Cell Line - CHO-K1?

A: When conducting gene editing with Human OKT3 scFv Stable Cell Line - CHO-K1, it is important to maintain the expression and function of OKT3 scFv. Additionally, the choice and design of gene editing tools, such as CRISPR/Cas9, should ensure specificity to the target gene and accurate validation of the editing effects.

What are the prospects of the Human OKT3 scFv Stable Cell Line - CHO-K1 in cell communication research?

A: The Human OKT3 scFv Stable Cell Line - CHO-K1 has promising applications in studying cell communication, especially the interactions between immune cells. This cell line can be used to investigate the interactions between T-cell receptors and antigen-presenting cells, providing an important tool for understanding intercellular communication in immune responses.

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