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Cat.No. : CSC-RO0855 Host Cell: CT26
| Cat. No. | CSC-RO0855 |
| Description | This cell line is engineered to stably overexpress human NT5E (also named as CD73). |
| Gene | NT5E |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CT26 |
| Host Cell Species | Mus musculus (Mouse) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human NT5E Stable Cell Line - CT26 is generated by introducing the NT5E gene into CT26 cells using genetic engineering techniques, allowing them to stably express the NT5E protein. This typically involves cloning the NT5E gene into a suitable expression vector and then transfecting the vector into CT26 cells. Successfully transfected cells are then selected to form a cell line that stably expresses NT5E.
A: By modulating the expression levels of NT5E in the Human NT5E Stable Cell Line - CT26, its role in tumor growth and metastasis can be investigated. For example, RNA interference or CRISPR/Cas9 technology can be used to knock down or knockout the NT5E gene, and observe changes in cell growth, migration, and invasion abilities. In addition, NT5E can be expressed in various types of tumor cells to study its functions in different tumor contexts.
A: The Human NT5E Stable Cell Line - CT26 can be used for screening potential drugs targeting NT5E. For example, high-throughput screening methods can be employed to identify small molecule compounds that can inhibit NT5E activity or expression. These compounds can then be further studied for their anti-tumor activities, providing a foundation for the development of novel anti-tumor drugs.
A: To ensure the stability and activity of the Human NT5E Stable Cell Line - CT26 during experiments, strict aseptic techniques and appropriate culture conditions, such as temperature, humidity, and CO2 concentration, should be followed. Regular testing of the cells should be conducted to ensure they are free from contamination or genetic drift. Additionally, avoid excessive passaging of the cells to maintain their stability.
A: The advantages of using the Human NT5E Stable Cell Line - CT26 to study the interaction between NT5E and other signaling pathways include: 1) stable expression of NT5E, which facilitates the investigation of its role in signaling pathways; 2) the ability to observe the effects of NT5E on signaling pathways at the cellular level, revealing its functions in biological processes; 3) the possibility to further study the interaction between NT5E and other members of signaling pathways using gene editing technologies in this cell line.
A: When selecting control groups for experiments using the Human NT5E Stable Cell Line - CT26, consider the following strategies: 1) use untransfected CT26 cells as controls to exclude the effects of other factors on the experimental results; 2) use CT26 cells transfected with an empty vector as controls to evaluate the contribution of NT5E expression to the results; 3) if studying the role of NT5E under specific conditions, other stable cell lines with similar characteristics can be used as controls.
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