Human NT5E HEK293 Cell Lysate

Name: Human NT5E HEK293 Cell Lysate
SKU: CLOE-1113
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CLOE-1113 Host Cell : HEK293

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Cell Lysate Information

Gene Information

Cat. No.	CLOE-1113
Introduction	This cell lysate over-expresses C-terminal polyhistidine tagged human NT5E (NP_002517.1) (Met 1-Lys 547) without expressing the propeptide. Purification of target protein (s) from this cell lysate has been verified.
Target Gene	NT5E
Host Cell	HEK293
Host Cell Species	Homo sapiens (Human)
Applications	Positive control for Western Blot (WB), antibody verification, protein purification
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.
Media Type	Cells were cultured in DMEM supplemented with 10% fetal bovine serum.
Freeze Medium	Complete medium supplemented with 10% (v/v) DMSO
Storage	Upon receipt, store at 4°C, or prepare aliquots and store at -80°C. Multiple freeze-thaw cycles should be avoided.
Shipping	Ice package

Gene Name	NT5E 5-nucleotidase, ecto (CD73) [ Homo sapiens ]
Gene Symbol	NT5E
Synonyms	NT5E; 5-nucleotidase, ecto (CD73); 5 nucleotidase (CD73) , NT5; 5-nucleotidase; CD73; eN; eNT; 5-NT; ecto-5-nucleotidase; Purine 5-Prime-Nucleotidase; NT; NT5; NTE; E5NT;
Gene ID	4907
Uni Prot ID	P21589
m RNA Refseq	BC015940
Chromosome Location	6q14-q21
Function	5-nucleotidase activity; ferrous iron binding; hydrolase activity, acting on ester bonds; metal ion binding; nucleotide binding;
Pathway	HIF-1-alpha transcription factor network, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Nicotinate and nicotinamide metabolism, organism-specific biosystem; Nicotinate and nicotinamide metabolism, conserved biosystem; Purine catabolism, organism-specific biosystem;
MIM	129190

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