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Cat.No. : CSC-RO0085 Host Cell: HEK293T
| Cat. No. | CSC-RO0085 |
| Description | HEK293T-NT5E cell line is a stably transfected cell line which expresses human 5'-nucleotidase ecto (NT5E). |
| Gene | NT5E |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human NT5E Stable Cell Line-HEK293T is created by stably transfecting the human 5'-nucleotidase (NT5E) gene into HEK293T cells. This modification enables the cell line to efficiently express the NT5E protein.
A: This cell line can be used for screening drugs that may affect NT5E activity, particularly in the context of cancer treatment and inflammation control.
A: The stability is maintained by culturing in selective media containing antibiotics that select and maintain the expression of the NT5E gene.
A: Due to specific genetic modification, this cell line is highly efficient in expressing NT5E protein, suitable for high-throughput protein production.
A: It is particularly useful in cancer biology, immunology, cell signaling, and drug discovery research.
A: The functional activity of NT5E protein can be verified by enzyme activity assays and Western blotting.
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