Human NT5E Stable Cell Line-HEK293T

Name: Human NT5E Stable Cell Line-HEK293T
SKU: CSC-RO0085
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0085

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0085
Description	HEK293T-NT5E cell line is a stably transfected cell line which expresses human 5'-nucleotidase ecto (NT5E).
Target Gene	NT5E
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	NT5E 5-nucleotidase, ecto (CD73) [ Homo sapiens ]
Gene Symbol	NT5E
Synonyms	NT5E; 5-nucleotidase, ecto (CD73); 5 nucleotidase (CD73) , NT5; 5-nucleotidase; CD73; eN; eNT; 5-NT; ecto-5-nucleotidase; Purine 5-Prime-Nucleotidase; NT; NT5; NTE; E5NT;
Gene ID	4907
Uni Prot ID	P21589
m RNA Refseq	BC015940
Chromosome Location	6q14-q21
Function	5-nucleotidase activity; ferrous iron binding; hydrolase activity, acting on ester bonds; metal ion binding; nucleotide binding;
Pathway	HIF-1-alpha transcription factor network, organism-specific biosystem; Metabolic pathways, organism-specific biosystem; Metabolism, organism-specific biosystem; Metabolism of nucleotides, organism-specific biosystem; Nicotinate and nicotinamide metabolism, organism-specific biosystem; Nicotinate and nicotinamide metabolism, conserved biosystem; Purine catabolism, organism-specific biosystem;
MIM	129190

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Q & A

Customer Reviews

Customer Q&As

What specific genetic modification is made in the Human NT5E Stable Cell Line-HEK293T?

A: The Human NT5E Stable Cell Line-HEK293T is created by stably transfecting the human 5'-nucleotidase (NT5E) gene into HEK293T cells. This modification enables the cell line to efficiently express the NT5E protein.

What is the application of this cell line in drug screening?

A: This cell line can be used for screening drugs that may affect NT5E activity, particularly in the context of cancer treatment and inflammation control.

How is the stability of the Human NT5E Stable Cell Line-HEK293T maintained?

A: The stability is maintained by culturing in selective media containing antibiotics that select and maintain the expression of the NT5E gene.

How efficient is this cell line in expressing NT5E?

A: Due to specific genetic modification, this cell line is highly efficient in expressing NT5E protein, suitable for high-throughput protein production.

In which research areas is the Human NT5E Stable Cell Line-HEK293T particularly useful?

A: It is particularly useful in cancer biology, immunology, cell signaling, and drug discovery research.

How to verify the functional activity of NT5E protein in the Human NT5E Stable Cell Line-HEK293T?

A: The functional activity of NT5E protein can be verified by enzyme activity assays and Western blotting.

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