Human MAS1-EGFP Stable Cell Line - HEK293

The MAS1 gene, also referred to as MAS or MGRA, encodes a class I seven-transmembrane G-protein-coupled receptor (GPCR) that functions as the primary receptor for angiotensin-(1-7), a key peptide within the protective arm of the renin-angiotensin system. Endogenously expressed in various tissues including the brain, heart, kidney, and vasculature, MAS1 preferentially couples to the Gq protein and activates the phospholipase C signaling cascade, playing central roles in blood pressure regulation, smooth muscle relaxation, anti-inflammatory responses, and cardioprotection. The Human MAS1-EGFP Stable Cell Line - HEK293 is a genetically engineered cell line derived from HEK293 cells that stably expresses full-length human MAS1 fused at its C-terminus to enhanced green fluorescent protein (EGFP). This allows for real-time visualization of receptor expression, subcellular localization, and trafficking dynamics in live cells without the need for antibody-based detection. The stable integration ensures consistent and reproducible expression levels across passages, making it a reliable tool for long-term experimental workflows.

This cell line is particularly valuable for GPCR research focused on the ACE2/Ang(1-7)/MAS1 axis, which has gained significant attention in cardiovascular biology, neuroprotection, and metabolic disease. Researchers can use the line for ligand binding and functional assays to characterize MAS1 agonists and antagonists, for studying receptor internalization and desensitization mechanisms via fluorescence microscopy or high-content imaging, and for investigating downstream signaling pathways such as PLC activation, calcium mobilization, and MAPK/ERK phosphorylation. The EGFP tag also enables fluorescence-based applications including flow cytometry for quantification of cell surface receptor levels, co-localization studies with organelle markers, and fluorescence resonance energy transfer (FRET)-based protein-protein interaction analysis. Additionally, the line serves as a controlled cellular system for evaluating the crosstalk between MAS1 and other components of the renin-angiotensin system, such as ACE2 and the AT1 receptor, in disease-relevant contexts including hypertension, cardiac remodeling, fibrosis, and neuroinflammation.