Human KLRD1 Stable Cell Line - CHO-K1

Name: Human KLRD1 Stable Cell Line - CHO-K1
SKU: CSC-RO0905
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO0905

Host Cell : CHO-K1 Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO0905
Description	This cell line is engineered to stably overexpress human KLRD1 (also named as CD94).
Target Gene	KLRD1
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	KLRD1 killer cell lectin-like receptor subfamily D, member 1 [ Homo sapiens ]
Gene Symbol	KLRD1
Synonyms	CD94
Gene Description	killer cell lectin-like receptor subfamily D, member 1
Gene ID	3824
Uni Prot ID	Q13241
m RNA Refseq	NM_007334.2
Protein Refseq	NP_031360.1
Chromosome Location	12p13
Function	carbohydrate binding; transmembrane signaling receptor activity;
Pathway	Adaptive Immune System, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; DAP12 interactions, organism-specific biosystem; DAP12 signaling, organism-specific biosystem; Graft-versus-host disease, organism-specific biosystem; Graft-versus-host disease, conserved biosystem;
MIM	602894

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Q & A

Customer Reviews

Customer Q&As

What is the specific expression pattern of Human KLRD1 Stable Cell Line - CHO-K1?

A: The Human KLRD1 Stable Cell Line - CHO-K1 expresses the human KLRD1 gene, which encodes the protein CD94, primarily active on the surface of Natural Killer (NK) cells. In the CHO-K1 cell line, the expression of the KLRD1 gene is maintained through stable transfection and selective culture media, ensuring its specificity and stability.

How adaptable is Human KLRD1 Stable Cell Line - CHO-K1 for studying NK cell functions?

A: This cell line is highly adaptable as a tool for studying NK cell functions. It stably expresses CD94 protein, which is related to NK cell activity, and can be used to study the activation, inhibition mechanisms of NK cells, and their interactions with tumor cells.

What is the potential application of Human KLRD1 Stable Cell Line - CHO-K1 in drug screening?

A: Human KLRD1 Stable Cell Line - CHO-K1 has significant potential in drug screening, especially in finding drugs that can modulate NK cell activity. Its stable expression of proteins related to NK cell function makes it an ideal model for assessing how drugs affect NK cell activity.

How can the gene editing efficiency of Human KLRD1 Stable Cell Line - CHO-K1 be evaluated?

A: The gene editing efficiency of this cell line can be assessed by monitoring changes in KLRD1 gene and protein expression through techniques such as quantitative PCR (qPCR) and Western blot. By comparing expression levels before and after editing, the editing efficiency can be effectively evaluated.

What role does Human KLRD1 Stable Cell Line - CHO-K1 play in immunotherapy research?

A: This cell line plays a key role in immunotherapy research. By simulating key characteristics of NK cells, it can be used to study the impact of different immunotherapy strategies, such as immune checkpoint inhibitors, on NK cell activity, thereby aiding in the development of new immunotherapy methods.

How does Human KLRD1 Stable Cell Line - CHO-K1 contribute to cell signaling research?

A: Human KLRD1 Stable Cell Line - CHO-K1 is very useful in studying signaling pathways related to NK cell activity. Researching the activation and inhibition mechanisms of KLRD1/CD94 can lead to a better understanding of how NK cells respond to immune challenges.

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