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Human KLRD1 Stable Cell Line - HEK293T

For research use only. Not intended for any clinical use.

Cat. No. :   CSC-RO0886

Host Cell :   HEK293T Size :   >1x106 frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No. CSC-RO0886
Description This cell line is engineered to stably overexpress human KLRD1 (also named as CD94).
Target Gene KLRD1
Gene Species Homo sapiens (Human)
Host Cell HEK293T
Host Cell Species Homo sapiens (Human)
Applications

1. Studying the interactions between immune cells and cancer cells

2. Studying the mechanisms of resistance to immune checkpoint blockade

3. High-throughput screening

4. Drug target validation

Size >1x106 frozen cells/vial
Stability Validated for at least 10 passages
Quality Control Negative for bacteria, yeast, fungi and mycoplasma.
Storage Liquid nitrogen
Shipping Dry ice
Revival Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6.
Mycoplasma Negative
Format One frozen vial containing millions of cells
Storage Liquid nitrogen
Safety Considerations The following safety precautions should be observed.
1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum.
2. No eating, drinking or smoking while handling the stable line.
3. Wash hands after handling the stable line and before leaving the lab.
4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells.
5. All waste should be considered hazardous.
6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship Dry ice
Gene Name KLRD1 killer cell lectin-like receptor subfamily D, member 1 [ Homo sapiens ]
Gene Symbol KLRD1
Synonyms CD94
Gene Description killer cell lectin-like receptor subfamily D, member 1
Gene ID 3824
Uni Prot ID Q13241
m RNA Refseq NM_007334.2
Protein Refseq NP_031360.1
Chromosome Location 12p13
Function carbohydrate binding; transmembrane signaling receptor activity;
Pathway Adaptive Immune System, organism-specific biosystem; Antigen processing and presentation, organism-specific biosystem; Antigen processing and presentation, conserved biosystem; DAP12 interactions, organism-specific biosystem; DAP12 signaling, organism-specific biosystem; Graft-versus-host disease, organism-specific biosystem; Graft-versus-host disease, conserved biosystem;
MIM 602894
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