Human IL6R Stable Cell Line-CHO-K1(GS-/-)

Name: Human IL6R Stable Cell Line-CHO-K1(GS-/-)
SKU: CSC-RO1053
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RO1053

Host Cell : CHO-K1(GS-/-) Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RO1053
Description	CHO-K1(GS-/-)-IL6R cell line is engineered to stably overexpress human IL6R.
Target Gene	IL6R
Gene Species	Homo sapiens (Human)
Host Cell	CHO-K1(GS-/-)
Host Cell Species	Cricetulus griseus (Chinese hamster)
Applications	1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival

Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	IL6R interleukin 6 receptor [ Homo sapiens ]
Gene Symbol	IL6R
Synonyms	IL6R; interleukin 6 receptor; interleukin-6 receptor subunit alpha; CD126; IL-6R 1; CD126 antigen; membrane glycoprotein 80; IL-6 receptor subunit alpha; gp80; IL6RA; IL-6RA; IL-6R-1; MGC104991;
Gene ID	3570
Uni Prot ID	P08887
m RNA Refseq	BC132686
Chromosome Location	1q21
Function	ciliary neurotrophic factor binding; contributes_to ciliary neurotrophic factor receptor activity; enzyme binding; interleukin-6 binding; contributes_to interleukin-6 receptor activity; contributes_to interleukin-6 receptor binding; protein binding; protein homodimerization activity; receptor activity;
Pathway	Cytokine Signaling in Immune system, organism-specific biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; IL-6 Signaling Pathway, organism-specific biosystem; IL6-mediated signaling events, organism-specific biosystem;
MIM	147880

Quick Inquiry

Q & A

Customer Reviews

Customer Q&As

What are the advantages of Human IL6R Stable Cell Line-CHO-K1(GS-/-)?

A: GS-/- CHO cell line expressing Human IL6R has the advantages of high yield, good stability, and high comparability, meeting the drug regulatory requirements of global biopharmaceutical companies.

How is Human IL6R Stable Cell Line-CHO-K1(GS-/-) related to the CHO cell line?

A: GS-/- CHO cell line has knocked out the glutamine synthetase (GS) gene. The transfected plasmid vector has the target gene and GS gene marker. When glutamine synthetase provides energy during ATP hydrolysis, it uses ammonia and glutamate in the cell to synthesize glutamine, providing nutrition for cell growth.

Has Human IL6R Stable Cell Line-CHO-K1(GS-/-) undergone quality control and certification to ensure consistency?

A: Yes.

Can Human IL6R Stable Cell Line-CHO-K1(GS-/-) be frozen and thawed multiple times without losing viability?

A: It can be repeatedly frozen and thawed.

What are the culture conditions for Human IL6R Stable Cell Line-CHO-K1(GS-/-)?

A: Compared to most cells, this cell line requires additional proline to grow.

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