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Cat.No. : CSC-RO0943 Host Cell: HeLa
| Cat. No. | CSC-RO0943 |
| Description | This cell line is engineered to stably overexpress human ICAM1. |
| Gene | ICAM1 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HeLa |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: CAM-1 belongs to the immunoglobulin superfamily and is involved in important physiological processes such as cell signal transduction and activation, immune response, and inflammation.
A: Human ICAM1 Stable Cell Line - HeLa uses Hela cells, which proliferate abnormally quickly.
A: It has been confirmed that HeLa cell lines are difficult to control. This cell line sometimes contaminates other cell cultures in the same laboratory, affecting biological research.
A: No, the publication of the Hela cell genome caused controversy, but it did not involve the use of this cell line.
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