Icam1 Knockout Mouse Spleen Cells

Name: Icam1 Knockout Mouse Spleen Cells
SKU: CSC-RK0100
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RK0100

Target Gene : Icam1 Host Cell : Spleen cells

Size : >1x10⁶ cells/vial Validation : Sequencing

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RK0100
Background	ICAM-1 (Inter-Cellular Adhesion Molecule 1) also known as CD54 (Cluster of Differentiation 54) is a protein that in humans is encoded by the ICAM1 gene. This gene encodes a cell surface glycoprotein which is typically expressed on endothelial cells and cells of the immune system. It binds to integrins of type CD11a/CD18, or CD11b / CD18 and is also exploited by rhinovirus as a receptor. From Wikipedia, the free encyclopedia.
Target Gene	Icam1
Abbr	MPSPLCs-Icam1 Knockout
Alias	ICAM1, myD10, CD54, Ly-47, Icam-1, MALA-2, MGC6195
Host Cell	Spleen cells
Host Cell Species	Mus musculus (Mouse)
Size	>1x10⁶ cells/vial
Validation	Sequencing
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Adherent

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	Icam1 intercellular adhesion molecule 1 [ Mus musculus ]
Gene Symbol	Icam1
Synonyms	CD54; Ly-47; Icam-1; MALA-2
Gene Description	intercellular adhesion molecule
Gene ID	15894
Uni Prot ID	P13597
m RNA Refseq	NM_010493.2
Protein Refseq	NP_034623.1
Chromosome Location	9 A3; 9 7.0 cM
Function	cell surface binding; integrin binding; protein complex binding;
Pathway	Adaptive Immune System, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem; African trypanosomiasis, conserved biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; Epstein-Barr virus infection, organism-specific biosystem;

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Q & A

Customer Reviews

Customer Q&As

How is the knockout cell line validated?

A: The knockout cell product is validated by PCR amplification and Sanger Sequencing to confirm the mutation at the genomic level. Please find the detailed mutation info in the datasheet.

Is the product a single clonal cell or mixed cell pool?

A: Single clonal cell.

Can I confirm gene knockout by RT-qPCR?

A: No. This knockout cell product is generated using the CRISPR/Cas9 system to induce small insertions or deletions (indels) resulting in frameshift mutations. Although these frameshift mutations typically disrupt the coding gene, there is a possibility that the non-functional transcript may still be transcribed. Consequently, this could potentially yield misleading results when analyzed by RT-qPCR.

How can I store the cell product?

A: The cell line should be stored in liquid nitrogen for long-term preservation.

Is it possible to get multiple knockout clones for my GOI?

A: For most cases, we often keep at least 2 clones with different frameshift mutations. Please feel free to contact us to check if there are additional available clones.

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