Human GPRC5B Stable Cell Line - HEK293T

Name: Human GPRC5B Stable Cell Line - HEK293T
SKU: CSC-RG1809
Availability: InStock

For research use only. Not intended for any clinical use.

Cat. No. : CSC-RG1809

Host Cell : HEK293T Size : >1x10⁶ frozen cells/vial

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Cell Line Information

Cell Culture Information

Safety and Packaging

Gene Information

Cat. No.	CSC-RG1809
Description	This cell line is engineered to stably overexpress Human GPRC5B in HEK293T cells.
Target Gene	GPRC5B
Gene Species	Homo sapiens (Human)
Host Cell	HEK293T
Host Cell Species	Homo sapiens (Human)
Applications	1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases
Size	>1x10⁶ frozen cells/vial
Stability	Validated for at least 10 passages
Quality Control	Negative for bacteria, yeast, fungi and mycoplasma.
Storage	Liquid nitrogen
Shipping	Dry ice

Revival	Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media.
Growth Properties	Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO₂. Split at 80-90% confluence, approximately 1:3-1:6.

Mycoplasma	Negative
Format	One frozen vial containing millions of cells
Storage	Liquid nitrogen
Safety Considerations	The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach.
Ship	Dry ice

Gene Name	GPRC5B G protein-coupled receptor, family C, group 5, member B [ Homo sapiens ]
Gene Symbol	GPRC5B
Synonyms	GPRC5B; G protein-coupled receptor, family C, group 5, member B; G protein coupled receptor, family C, group 1, member B; G-protein coupled receptor family C group 5 member B; RAIG 2; A-69G12.1; retinoic acid-induced gene 2 protein; retinoic acid responsive gene protein; G protein-coupled receptor, family C, group 1, member B; RAIG2; RAIG-2;
Gene ID	51704
Uni Prot ID	Q9NZH0
m RNA Refseq	BC034467
Chromosome Location	16p12
Function	G-protein coupled receptor activity; receptor activity; signal transducer activity;
Pathway	GPCRs, Class C Metabotropic glutamate, pheromone, organism-specific biosystem;
MIM	605948

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