Transfected Stable Cell Lines
Reliable | High-Performance | Wide Rage
Precision reporter, kinase, immune receptor, biosimilar, Cas9, and knockout stable cell lines for diverse applications.
Cat. No. : CSC-RG1825
Host Cell : HEK293 Size : >1x106 frozen cells/vial
| Cat. No. | CSC-RG1825 |
| Description | This cell line is engineered to stably overexpress human G protein-coupled receptor 84 (GPR84) in HEK293. |
| Target Gene | GPR84 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293 |
| Host Cell Species | Homo sapiens (Human) |
| Applications |
1. Gene expression studies 2. Signaling pathway research 3. Drug screening and toxicology 4. Research on the mechanisms of GPCR-related diseases |
| Size | >1x106 frozen cells/vial |
| Stability | Validated for at least 10 passages |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Storage | Liquid nitrogen |
| Shipping | Dry ice |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Media Type | Cells were cultured in DMEM supplemented with 10% fetal bovine serum. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Freeze Medium | Complete medium supplemented with 10% (v/v) DMSO |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
| Gene Name | GPR84 G protein-coupled receptor 84 [ Homo sapiens ] |
| Gene Symbol | GPR84 |
| Synonyms | GPR84; G protein-coupled receptor 84; G-protein coupled receptor 84; EX33; inflammation-related G protein-coupled receptor EX33; inflammation-related G-protein coupled receptor EX33; GPCR4; |
| GeneID | 53831 |
| Uni ProtID | Q9NQS5 |
| mRNA Refseq | BC020614 |
| Chromosome Location | 12q13.13 |
| Function | G-protein coupled receptor activity; receptor activity; signal transducer activity; |
| Pathway | GPCRs, Other, organism-specific biosystem; |
| MIM | 606383 |
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This GPR84 stable cell line provided a convenient starting point for our receptor research. The cells recovered satisfactorily and were compatible with our established HEK293 culture workflow. We appreciated the stable-expression format, which supported efficient investigation of GPR84 signaling and pharmacological responses.
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