Human GLP1R/CRE-Luc Stable Cell Line - HEK293

The human GLP1R gene encodes the glucagon-like peptide-1 receptor (GLP-1R), a Class B G protein-coupled receptor activated by GLP-1 and related incretin agonists. Receptor activation is primarily mediated by Gs proteins, stimulating adenylyl cyclase activity and increasing intracellular cAMP levels, thereby driving glucose-dependent insulin secretion signaling in physiological tissues. This product is a genetically engineered, stable HEK293 reporter cell line that co-expresses human GLP1R under the control of a CMV promoter and contains a luciferase reporter gene regulated by a cAMP response element (CRE). HEK293 cells offer a well-characterized human background, robust growth, and highly reproducible exogenous gene expression, while being widely compatible with microplate-based biochemical and imaging workflows. By coupling receptor activation to a transcriptional bioluminescent output, this cell line converts accumulated cAMP/CRE signaling into a scalable and easily quantifiable detection signal.

Functionally, under optimized assay conditions, GLP1R agonism enhances cAMP-dependent CRE activity and luciferase output, whereas receptor antagonism or inhibition of pathway components reduces the induced signal. This cell line is suitable for concentration-response analysis, potency ranking, agonist and antagonist screening, comparison of peptide or small-molecule modulators, and—in conjunction with orthogonal assays—the study of receptor-biased or pathway-selective pharmacology. Its bioluminescent readout supports medium- to high-throughput workflows, assay development, lot-to-lot comparisons, and preliminary structure-activity relationship studies.