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Cat.No. : CSC-RO1050 Host Cell: HEK293T
| Cat. No. | CSC-RO1050 |
| Description | HEK293-CD226 cell line is engineered to stably overexpress human CD226. |
| Gene | CD226 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | HEK293T |
| Host Cell Species | Homo sapiens (Human) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Growth Properties | Cells are cultured as a monolayer at 37°C in a humidified atmosphere with 5% CO2. Split at 80-90% confluence, approximately 1:3-1:6. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: The Human CD226 Stable Cell Line-HEK293 originates from human embryonic kidney 293 (HEK293) cells that were transfected with a stable expression construct containing the human CD226 (DNAM-1) gene. CD226 is a cell surface receptor involved in immune cell activation and is associated with natural killer (NK) cell function. The cell line was established through a transfection and selection process to create stable cell populations expressing human CD226.
A: The Human CD226 Stable Cell Line-HEK293 is primarily used in research related to immunology, particularly in the study of immune cell interactions and signaling pathways. It serves as a valuable tool for investigating the binding of CD226 to its ligands, such as CD155 (PVR) and CD112 (Nectin-2), and understanding how CD226 signaling influences immune responses. Researchers also use this cell line to explore potential immunotherapies targeting CD226.
A: CD226 (DNAM-1) is known to interact with its ligands CD155 (PVR) and CD112 (Nectin-2), which are expressed on target cells. These interactions are crucial for immune cell recognition and cytotoxicity, particularly in NK cell-mediated responses. Targeting CD226 or its ligands has been explored in immunotherapy to enhance immune cell-mediated killing of cancer cells.
A: When using the Human CD226 Stable Cell Line-HEK293 in research involving human subjects or clinical applications, researchers must adhere to ethical guidelines, obtain appropriate informed consent when working with patient-derived samples, and ensure compliance with institutional review board (IRB) or ethics committee requirements. Additionally, ethical considerations related to genetic manipulation and potential therapeutic applications should be carefully evaluated.
A: The Human CD226 Stable Cell Line-HEK293 has allowed researchers to investigate the specific interactions and signaling pathways involving CD226 in a controlled environment. This cell line has contributed to our understanding of CD226 biology and its role in immune cell activation, providing insights that can inform the development of targeted immunotherapies.
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