Cat.No. : CSC-RO1049 Host Cell: CHO-S
| Cat. No. | CSC-RO1049 |
| Description | CHO-S-CD226 cell line is engineered to stably overexpress human CD226. |
| Gene | CD226 |
| Gene Species | Homo sapiens (Human) |
| Host Cell | CHO-S |
| Host Cell Species | Cricetulus griseus (Chinese hamster) |
| Stability | Validated for at least 10 passages |
| Application | 1. Studying the interactions between immune cells and cancer cells 2. Studying the mechanisms of resistance to immune checkpoint blockade 3. High-throughput screening 4. Drug target validation |
| Quality Control | Negative for bacteria, yeast, fungi and mycoplasma. |
| Shipping | Dry ice |
| Storage | Liquid nitrogen |
| Revival | Rapidly thaw cells in a 37°C water bath. Transfer contents into a tube containing pre-warmed media. Centrifuge cells and seed into a 25 cm2 flask containing pre-warmed media. |
| Mycoplasma | Negative |
| Format | One frozen vial containing millions of cells |
| Storage | Liquid nitrogen |
| Safety Considerations |
The following safety precautions should be observed. 1. Use pipette aids to prevent ingestion and keep aerosols down to a minimum. 2. No eating, drinking or smoking while handling the stable line. 3. Wash hands after handling the stable line and before leaving the lab. 4. Decontaminate work surface with disinfectant or 70% ethanol before and after working with stable cells. 5. All waste should be considered hazardous. 6. Dispose of all liquid waste after each experiment and treat with bleach. |
| Ship | Dry ice |
A: Human CD226 Stable Cell Line-CHO-S is a cell line engineered to express the CD226 antigen, which is a member of the immunoglobulin superfamily and plays a role in cell adhesion and immune cell activation. This cell line is particularly useful for studying the interactions between CD226 and its ligands, such as DNAM-1 (CD226), which is crucial for the activation of natural killer (NK) cells and T cells. It can be used to investigate the molecular mechanisms of immune cell activation and to develop therapeutic strategies for immune-related diseases.
A: The Human CD226 Stable Cell Line-CHO-S is specifically engineered to express the CD226 protein, which is not typically found in standard CHO cell lines. This makes it a unique resource for the production of recombinant proteins or antibodies targeting CD226, which can be critical for the development of new therapeutics. Additionally, the stable expression of CD226 in this cell line allows for consistent and high-level production of the protein, which is essential for biopharmaceutical applications.
A: To maintain the stability of the Human CD226 Stable Cell Line-CHO-S, it is crucial to follow optimized culture conditions, including the use of appropriate growth media, temperature, and CO2 levels. Regular monitoring for mycoplasma contamination and periodic testing for the expression of CD226 are also necessary to ensure the cell line retains its intended characteristics. Additionally, cryopreservation and proper thawing procedures are essential to prevent loss of CD226 expression during cell passage.
A: The Human CD226 Stable Cell Line-CHO-S can be used to screen for potential immunotherapeutic agents that modulate the activity of CD226. This can involve testing the effects of small molecules, antibodies, or other biologics on the expression and function of CD226. The cell line can also be used to generate CD226-specific antibodies for use in immunoassays or as therapeutic agents.
A: The Human CD226 Stable Cell Line-CHO-S provides a model system to study the role of CD226 in immune cell development and differentiation. By manipulating the expression levels of CD226 or its ligands, researchers can investigate the impact on cell proliferation, differentiation, and immune response. This can lead to insights into the molecular pathways regulated by CD226 and its implications for immune cell function.
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