CMV-SV40/LTA(RFP, Puro) Lentiviral Particles

The CMV-SV40/LTA (RFP, Puro) Lentiviral Particles represent a sophisticated tool for the stable delivery and high-level expression of the Simian Virus 40 Large T Antigen (SV40 LTA) in a wide variety of mammalian host cells. These third-generation lentiviral particles are engineered for safety and efficiency, utilizing the human cytomegalovirus (CMV) constitutive promoter to drive the expression of the SV40 LTA gene. To streamline experimental workflows, the construct is dual-labeled with Red Fluorescent Protein (RFP), allowing for non-invasive monitoring of transduction efficiency via fluorescence microscopy, and a Puromycin resistance marker (Puro) for the rapid selection of stable cell lines. The VSV-G pseudotyping ensures high-titer delivery into both dividing and quiescent cells, making this system significantly more versatile than traditional plasmid-based transfection methods, especially when working with difficult-to-transduce primary cells or non-dividing populations.

The primary application of these lentiviral particles lies in the immortalization of primary cells to create continuous cell lines, which are indispensable for longitudinal studies in cell biology and drug discovery. By neutralizing the p53 and Retinoblastoma (Rb) pathways, the SV40 Large T Antigen allows cells to circumvent the Hayflick limit and bypass replicative senescence. This capability is crucial for researchers working with rare or finite primary cell samples who require a consistent and reproducible cellular model for long-term experimentation. Furthermore, these particles are extensively used in oncogenesis research to study the early stages of malignant transformation and to evaluate the role of viral oncogenes in perturbing host cell cycles. The combined RFP and Puromycin features make them particularly well-suited for complex assays, such as lineage tracing in heterogeneous cell populations or developing standardized substrates for large-scale pharmaceutical screening, ensuring both phenotypic stability and easy quantification.