Caspase-9 Activity Assay Kit

Name: Caspase-9 Activity Assay Kit
SKU: PTAK-010

For research use only. Not intended for any clinical use.

Cat.No.

PTAK-010

Description

This kit provides a convenient means for detecting activated caspase-9 in living cells. The assay utilizes a caspase-9 inhibitor LEHD-FMK conjugated to sulfo-rhodamine (Red-LEHD-FMK) as the fluorescent marker. Red-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells

Applications

Useful for detecting the activity of caspase-9 in living cells.

Caution

Avoid freeze/thaw cycles

Component

•Caspase-9
•Caspase-9 assay buffer
•Caspase-9 substrate (Red-LEHD-FMK)
•Caspase-9 inhibitor (Z-VAD-FMK)
•DTT

Method

Fluorescence

Size/Form

100 assays

Publications

Q & A

Customer Reviews

Customer Q&As

What is Caspase-9?

A: Caspase-9 is an enzyme encoded by the CASP9 gene in humans. It is an initiating caspase that is essential for the apoptotic pathway found in many tissues.

What is the structure of Caspase-9?

A: Similar to other caspases, caspase-9 has three domains: an N-terminal pro-domain, a large subunit, and a small subunit.

How does caspase-9 cause apoptosis?

A: When a cell receives signals to undergo apoptosis, caspase-9 is activated through the formation of a protein complex called the apoptosome. Once activated, caspase-9 cleaves and activates downstream effector caspases, such as caspase-3 and caspase-7. These effector caspases then cleave various cellular proteins, leading to the breakdown of the cell's components and ultimately cell death.

How does caspase-9 get activated?

A: In dying cells, activation of caspase-9 is mediated by a multimeric adapter complex called the Apaf-1 apoptosome. The apoptosome is formed by binding of cytochrome c released from mitochondria, ATP, and Apaf-1, which results in the activation of caspase-9.

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