Caspase-7 Activity Assay Kit

Name: Caspase-7 Activity Assay Kit
SKU: PTAK-008

For research use only. Not intended for any clinical use.

Cat.No.

PTAK-008

Description

The Caspase-7 Assay Kit is a complete assay system designed to measure Caspase-7 activity for screening and profiling applications. It comes in a convenient 96-well format, with all the reagents necessary for 100 fluorescent Caspase-7 activity measurements. In addition, the kit includes purified Caspase-7 enzyme and a potent Caspase-3/7 inhibitor, Ac-DNLD-CHO, for use as a positive and negative control. Using this kit, only one simple step on a microtiter plate is needed to analyze the Caspase-7 activity level. The fluorogenic substrate, Ac-DEVD-AFC, is incubated with purified Caspase-7 and the enzymatic activity releases AFC fluorophore that can then be measured using a fluorescence reader.

Applications

Great for studying enzyme kinetics and HTS applications.

Caution

Avoid freeze/thaw cycles

Component

•Caspase-7
•Caspase-7 assay buffer
•Caspase-7 substrate
•DTT
•Caspase-7/3 inhibitor (Ac-DNAD-CHO)
•Microtiter plate

Method

Fluorescence

Size/Form

100 assays

Publications

Q & A

Customer Reviews

Customer Q&As

What is Caspase-7？

A: Caspase-7, also known as CASP7, is an enzyme that plays a key role in the process of programmed cell death, or apoptosis.

How is caspase-7 activated?

A: Caspase-7, like other caspases, is activated through a process called proteolytic cleavage.

How big is the protein caspase-7?

A: The protein caspase-7, also known as cysteine-aspartic acid protease 7, has a molecular weight of approximately 35 kDa (kilodaltons). It consists of 303 amino acids and is composed of two subunits.

Does caspase-7 cleave PARP?

A: Yes, caspase-7 is known to cleave PARP (Poly(ADP-ribose) polymerase) during apoptosis. Cleavage of PARP by caspase-7 is a characteristic feature of apoptotic cell death and is used as a marker for the activation of caspase-7 and the induction of apoptosis.

Why are Caspase-7 activity assays important?

A: Caspase-7 activity assays are valuable tools for studying the role of caspase-7 in apoptosis and its regulation in different cellular contexts or disease states, such as cancer and neurodegenerative diseases.

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